Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PSE7367_RS08935 Genome accession   NC_019701
Coordinates   2261173..2263200 (+) Length   675 a.a.
NCBI ID   WP_015165039.1    Uniprot ID   K9SIN8
Organism   Pseudanabaena sp. PCC 7367     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2256173..2268200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSE7367_RS08900 (Pse7367_1791) - 2256226..2256780 (+) 555 WP_015165034.1 glutathione S-transferase family protein -
  PSE7367_RS08905 (Pse7367_1792) - 2256941..2257633 (+) 693 WP_015165035.1 glutathione binding-like protein -
  PSE7367_RS08910 (Pse7367_1793) - 2257801..2258133 (+) 333 WP_015165036.1 hypothetical protein -
  PSE7367_RS08925 (Pse7367_1794) - 2258746..2259099 (+) 354 WP_015165037.1 rubredoxin -
  PSE7367_RS08930 (Pse7367_1795) - 2259174..2260241 (+) 1068 WP_156800368.1 photosynthesis system II assembly factor Ycf48 -
  PSE7367_RS08935 (Pse7367_1796) pilB/pilB1 2261173..2263200 (+) 2028 WP_015165039.1 GspE/PulE family protein Machinery gene
  PSE7367_RS08940 (Pse7367_1797) pilT 2263312..2264460 (+) 1149 WP_041699378.1 type IV pilus twitching motility protein PilT Machinery gene
  PSE7367_RS08945 (Pse7367_1798) - 2264574..2265776 (+) 1203 WP_015165041.1 type II secretion system F family protein -
  PSE7367_RS08950 (Pse7367_1799) miaA 2265720..2266676 (-) 957 WP_015165042.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  PSE7367_RS08955 (Pse7367_1800) nth 2267119..2267721 (-) 603 Protein_1775 endonuclease III -

Sequence

Protein


Download         Length: 675 a.a.        Molecular weight: 75645.75 Da        Isoelectric Point: 5.1414

>NTDB_id=54820 PSE7367_RS08935 WP_015165039.1 2261173..2263200(+) (pilB/pilB1) [Pseudanabaena sp. PCC 7367]
MNTSGQQKQGSTKNRKALLIRGGRTPFETKLIESGHATSEQYDRALEKSEEDEISLLAALQEVLNQALPPDIARYYKRLR
LLELKLLYGFECLDPDIDVEKFNISNISGLIETDVVPMSTCRNHEVLPLVKKDNTLLVGMVSPDNFKAIDDLSMLLSKQE
LTLQRRVITREDFNDLLEQIIEAQVNKSVKGESFDDEALRIDDDAFADETLGEVDEGDDLSKAIGENSAPVINLVDKILV
KALQEGASDIHIEPQEKNLRIRFRRDGVLREVFFLNDDRRLPKSITAAVIARFKIISNLNIAERRVPQDGKLKRVFQKRR
VDFRVSTCPSQHGEKVVLRILDNSNTQLGLDKLITDPESLEIMQGMAQRPFGLILVTGPTGSGKTTTLYSALAEVNEPGI
NISTAEDPVEYNLPGLTQCQVIRAKGMDFALILRAFLRQDPDVILVGETRDQETAKTAIEAALTGHLVLTTLHTNDAPSA
IARLEEMGVERFMASTALIGVLAQRLLRRVCDVCRIPYQPTIEDIERFGLPRTDLESTFYKANRLSPQEVIARQESNLSV
CKKCGGSGYKGRVGCYEIMRMSENMQVAINKGATTEMIKEIAVQEGMKTLMAYSFELVRTGATTLEEVLRVVYTDKGREA
EERAKRQNTLECQNCHAILKPEMIECPYCTTPRII

Nucleotide


Download         Length: 2028 bp        

>NTDB_id=54820 PSE7367_RS08935 WP_015165039.1 2261173..2263200(+) (pilB/pilB1) [Pseudanabaena sp. PCC 7367]
ATGAATACTTCAGGTCAGCAGAAACAAGGTAGCACCAAGAACCGTAAAGCTCTATTAATTAGAGGGGGGCGGACTCCATT
TGAAACTAAACTTATTGAGTCTGGACATGCTACTTCAGAGCAGTACGATCGCGCCCTTGAAAAGAGTGAAGAAGATGAGA
TTTCTTTGCTAGCAGCGCTTCAAGAGGTACTAAACCAAGCCCTACCACCTGATATTGCCCGCTATTACAAGCGGCTCCGT
TTGCTAGAACTGAAGTTGCTCTATGGCTTTGAATGTCTTGACCCTGATATTGATGTTGAAAAATTTAACATCAGTAATAT
TTCTGGGTTGATCGAAACCGATGTGGTGCCCATGTCCACCTGTCGAAACCATGAGGTTTTGCCACTGGTCAAAAAAGACA
ATACCCTGTTGGTGGGGATGGTCAGCCCTGATAACTTCAAGGCGATCGATGACCTGAGTATGCTATTGAGCAAGCAAGAA
TTGACCTTGCAACGCCGGGTAATCACCCGCGAAGATTTCAACGATCTGCTAGAGCAAATTATTGAAGCCCAGGTCAACAA
AAGTGTTAAGGGCGAAAGCTTTGATGATGAGGCGCTGCGGATCGATGATGATGCCTTTGCCGATGAGACCCTCGGTGAGG
TTGATGAAGGCGATGACCTGAGCAAGGCGATCGGCGAGAACTCTGCCCCAGTCATTAACCTGGTCGATAAAATATTAGTT
AAAGCACTCCAGGAAGGGGCTTCTGATATTCACATTGAACCCCAGGAGAAAAATTTGCGGATTCGCTTCCGCCGTGATGG
GGTGTTGCGGGAAGTATTTTTCCTCAATGACGATCGCCGCCTACCCAAGAGCATCACTGCCGCGGTAATCGCTCGCTTTA
AGATTATTTCCAACCTAAACATTGCGGAACGCCGGGTTCCCCAAGATGGCAAGCTCAAGCGCGTGTTCCAAAAGCGGCGG
GTTGACTTCCGGGTCAGCACTTGCCCCAGCCAACATGGCGAGAAAGTGGTGCTGCGGATTCTAGACAACTCCAACACTCA
GTTGGGGCTAGACAAGCTAATCACCGATCCAGAAAGTCTAGAGATCATGCAGGGTATGGCTCAGCGCCCCTTTGGTCTGA
TTCTGGTGACTGGGCCGACTGGTTCCGGTAAAACCACTACTCTGTATTCCGCTTTGGCAGAAGTTAATGAGCCAGGAATT
AACATTAGTACGGCGGAAGACCCGGTTGAATATAACCTGCCTGGACTGACTCAATGTCAGGTAATTCGTGCCAAGGGCAT
GGACTTTGCCTTGATCCTGCGGGCATTTTTGCGGCAGGACCCCGATGTAATTCTGGTGGGGGAAACCCGTGACCAAGAAA
CTGCTAAAACAGCGATCGAAGCAGCGTTAACGGGTCACCTGGTACTAACTACCCTGCACACCAATGATGCCCCCAGTGCG
ATCGCGCGGTTAGAAGAAATGGGCGTAGAGCGATTTATGGCCAGTACGGCGCTGATTGGTGTATTGGCACAACGCCTATT
GCGGCGGGTTTGTGATGTTTGCCGTATCCCCTATCAACCCACGATCGAGGATATTGAGCGCTTTGGTTTACCTCGCACCG
ATCTAGAAAGCACCTTCTATAAGGCCAATCGCCTTAGCCCCCAGGAAGTGATCGCCCGTCAGGAATCCAATCTATCTGTT
TGTAAGAAATGCGGCGGATCTGGGTATAAAGGACGGGTAGGTTGCTACGAAATTATGCGCATGAGCGAAAACATGCAGGT
GGCAATTAATAAGGGTGCGACCACCGAAATGATCAAGGAGATCGCAGTCCAGGAAGGGATGAAAACCCTGATGGCCTATA
GCTTTGAATTGGTCAGAACCGGTGCTACAACCCTGGAAGAAGTGTTGCGGGTGGTTTACACCGACAAAGGGCGAGAGGCA
GAGGAACGCGCCAAGCGCCAAAATACGCTGGAATGCCAGAACTGCCATGCAATTTTGAAACCAGAAATGATTGAATGTCC
CTATTGCACTACGCCAAGAATTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(237-509)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(109-184)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9SIN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

55.935

99.852

0.559

Multiple sequence alignment