Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW53_RS20470 Genome accession   NZ_CP071808
Coordinates   3976948..3977991 (-) Length   347 a.a.
NCBI ID   WP_002199361.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS014     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3971948..3982991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS20425 (EXW53_20480) - 3972348..3972548 (-) 201 WP_002015009.1 YqzE family protein -
  EXW53_RS20430 (EXW53_20485) - 3972587..3973084 (-) 498 WP_002111816.1 shikimate kinase -
  EXW53_RS20435 (EXW53_20490) - 3973236..3973886 (-) 651 WP_215557463.1 2OG-Fe(II) oxygenase -
  EXW53_RS20440 (EXW53_20495) comGG 3974059..3974430 (-) 372 WP_002199366.1 competence type IV pilus minor pilin ComGG -
  EXW53_RS20445 (EXW53_20500) comGF 3974427..3974888 (-) 462 WP_215557464.1 competence type IV pilus minor pilin ComGF -
  EXW53_RS20450 (EXW53_20505) - 3974872..3975168 (-) 297 WP_002111824.1 hypothetical protein -
  EXW53_RS20455 (EXW53_20510) comGD 3975161..3975616 (-) 456 WP_002199363.1 competence type IV pilus minor pilin ComGD -
  EXW53_RS20460 (EXW53_20515) comGC 3975613..3975912 (-) 300 WP_002111826.1 competence type IV pilus major pilin ComGC -
  EXW53_RS20465 (EXW53_20520) comGB 3975924..3976961 (-) 1038 WP_002199362.1 competence type IV pilus assembly protein ComGB -
  EXW53_RS20470 (EXW53_20525) comGA 3976948..3977991 (-) 1044 WP_002199361.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW53_RS20475 (EXW53_20530) - 3978197..3978892 (+) 696 WP_002199360.1 helix-turn-helix domain-containing protein -
  EXW53_RS20480 (EXW53_20535) - 3979024..3979266 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW53_RS20485 (EXW53_20540) - 3979374..3980768 (+) 1395 WP_002199359.1 L-cystine transporter -
  EXW53_RS20490 (EXW53_20545) - 3980849..3981259 (-) 411 WP_002199358.1 hypothetical protein -
  EXW53_RS20495 (EXW53_20550) - 3981329..3981544 (-) 216 WP_002199357.1 DUF3912 family protein -
  EXW53_RS20500 (EXW53_20555) - 3981797..3982108 (+) 312 WP_002199356.1 hypothetical protein -
  EXW53_RS20505 (EXW53_20560) - 3982142..3982630 (-) 489 WP_002111841.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39158.34 Da        Isoelectric Point: 8.2375

>NTDB_id=548021 EXW53_RS20470 WP_002199361.1 3976948..3977991(-) (comGA) [Bacillus mycoides strain JAS014]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQSSVQPLSHLSLFPSTAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGETRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDATGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIREASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=548021 EXW53_RS20470 WP_002199361.1 3976948..3977991(-) (comGA) [Bacillus mycoides strain JAS014]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAAAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAAAGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTAGTTATTCGCCT
CCATTTACAATCATCTGTTCAGCCGTTGTCTCATCTTTCGTTATTTCCAAGTACAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACGACAATGTATGCGTTATTAGAAGTA
GCTAGAAAAGGAGAAACACGTCGTATTGTTACTCTGGAAGATCCAGTTGAGAGAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATGAAACGGGATTAAAGGCTATTTTGCGTCACGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTGATGACAACCTTGCAT
ACAAATGATGCGACAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGACTCGTTGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGGCAGGCAAGTATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAGAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTGCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Haemophilus influenzae 86-028NP

39.13

92.795

0.363
  pilB Glaesserella parasuis strain SC1401

43.706

82.421

0.36