Detailed information NTG0548001

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: EXW53_RS05710
• Genome accession: NZ_CP071808
• Coordinates: 1116016..1119741 (+)
• Length: 1241 a.a.
• NCBI ID: WP_215558129.1
• Uniprot ID: -
• Organism: Bacillus mycoides strain JAS014
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1111016..1124741

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXW53_RS05695 (EXW53_05705)	-	1111174..1111767 (+)	594	WP_002201415.1	TVP38/TMEM64 family protein	-
EXW53_RS05700 (EXW53_05710)	lepB	1111823..1112386 (+)	564	WP_002158924.1	signal peptidase I	-
EXW53_RS05705 (EXW53_05715)	addB	1112504..1116019 (+)	3516	WP_215558128.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
EXW53_RS05710 (EXW53_05720)	addA	1116016..1119741 (+)	3726	WP_215558129.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
EXW53_RS05715 (EXW53_05725)	-	1119765..1120043 (+)	279	WP_002201412.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
EXW53_RS05720 (EXW53_05730)	-	1120260..1120967 (+)	708	WP_002201411.1	DNA alkylation repair protein	-
EXW53_RS05725 (EXW53_05735)	-	1121237..1121722 (+)	486	WP_002201410.1	hypothetical protein	-
EXW53_RS05730 (EXW53_05740)	gerPF	1121808..1122023 (-)	216	WP_001141570.1	spore germination protein GerPF	-
EXW53_RS05735 (EXW53_05745)	-	1122066..1122452 (-)	387	WP_002140807.1	spore germination protein GerPE	-
EXW53_RS05740 (EXW53_05750)	gerPD	1122467..1122661 (-)	195	WP_001052805.1	spore germination protein GerPD	-
EXW53_RS05745 (EXW53_05755)	-	1122668..1123282 (-)	615	WP_002158914.1	spore germination protein GerPC	-
EXW53_RS05750 (EXW53_05760)	gerPB	1123347..1123553 (-)	207	WP_002201409.1	spore germination protein GerPB	-
EXW53_RS05755 (EXW53_05765)	gerPA	1123568..1123789 (-)	222	WP_002158912.1	spore germination protein GerPA	-
EXW53_RS05760 (EXW53_05770)	-	1123886..1124065 (-)	180	WP_002087032.1	aspartyl-phosphate phosphatase Spo0E family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142733.95 Da
• Isoelectric Point: 4.8704

>NTDB_id=548001 EXW53_RS05710 WP_215558129.1 1116016..1119741(+) (addA) [Bacillus mycoides strain JAS014]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEEHI
RKATELAMLPDGPAPRVETLQADLALLRTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYKLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYGYSASWK
VEVVDGKTLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKSDESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPTL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=548001 EXW53_RS05710 WP_215558129.1 1116016..1119741(+) (addA) [Bacillus mycoides strain JAS014]
ATGATAGAAAATTGGCCTGAGAAACCAGAAGGTAGTCAATGGACAGATGATCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCGGCGGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAACAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACAATTCATTCATTTTGTTTGCAAGTTATTAGAGGGTATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGATGTTGAAGGAA
AGACGATTGAAGATTTGGTGTACGCTTCTTACCTATTAGAAGATGTAAAGTTTCAGCTTGAAACAGCGGAAGAGCATATT
CGTAAGGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTCGAAACGTTACAAGCAGATTTAGCTTTACT
TAGAACGTTATCATCAGCAGCTCGTGGGTCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCATGGCAAACGCTAA
AGCGTATTAAGAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTGGATTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAACGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGTGAGCAAAGTGAAGACGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCTGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTATGCA
TTCAGCAAACTGAGGAAGAAGTGCAAGAAGGTGAAGAAGGTACAGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCGGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCGCCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAAATTACTGCAAGGATGGCGTGAATTTGCGCGTCAACAATCTCTTTCCGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
ACGCGTATTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGATTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGACATGGGAACGGCGAGAGCTCTTGGAGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAGGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCATGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGGAATATTCCAGGCGAAATTTATGGGTATAGTGCGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAGTTATTAGAAGCGCTTCG
CGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGGAATATGGGTATGAGG
ACGCGACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTTCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAACGTGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGACGAATCGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAGACAGATACGATTGAAGGGAAGTTCCCAGGTGGATTTGATCAAGCGAAACCAACTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.649	100	0.539