Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW53_RS05705 Genome accession   NZ_CP071808
Coordinates   1112504..1116019 (+) Length   1171 a.a.
NCBI ID   WP_215558128.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS014     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1107504..1121019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS05670 (EXW53_05680) - 1108547..1109041 (-) 495 WP_002201417.1 DUF6584 family protein -
  EXW53_RS05675 (EXW53_05685) cspA 1109318..1109521 (+) 204 WP_000218989.1 RNA chaperone/antiterminator CspA -
  EXW53_RS05680 (EXW53_05690) - 1109641..1109826 (-) 186 WP_002011281.1 hypothetical protein -
  EXW53_RS05685 (EXW53_05695) - 1110107..1110184 (+) 78 Protein_1025 RNA-guided endonuclease TnpB family protein -
  EXW53_RS05690 (EXW53_05700) - 1110242..1110823 (+) 582 WP_002201416.1 competence protein ComK -
  EXW53_RS05695 (EXW53_05705) - 1111174..1111767 (+) 594 WP_002201415.1 TVP38/TMEM64 family protein -
  EXW53_RS05700 (EXW53_05710) lepB 1111823..1112386 (+) 564 WP_002158924.1 signal peptidase I -
  EXW53_RS05705 (EXW53_05715) addB 1112504..1116019 (+) 3516 WP_215558128.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW53_RS05710 (EXW53_05720) addA 1116016..1119741 (+) 3726 WP_215558129.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW53_RS05715 (EXW53_05725) - 1119765..1120043 (+) 279 WP_002201412.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW53_RS05720 (EXW53_05730) - 1120260..1120967 (+) 708 WP_002201411.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134275.42 Da        Isoelectric Point: 5.2129

>NTDB_id=548000 EXW53_RS05705 WP_215558128.1 1112504..1116019(+) (addB) [Bacillus mycoides strain JAS014]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMRLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYERIKQVAREEKIEFEKTIPLMGQPRFHSQALAHLEMHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVAAEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMTTGLEALQFANIPPSLDQVLVANIDHSRLSDIKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQALLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKETFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRMLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=548000 EXW53_RS05705 WP_215558128.1 1112504..1116019(+) (addB) [Bacillus mycoides strain JAS014]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTGAA
ACAACGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACACAGCAGGCGTTAATTGGGA
GTGAAGATGTAAGAGGTTCTATTCGGGCGCAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGG
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTCCATATGTTGCTTCGTAAAATTGTAGAATCTCGTAAAGATGGACT
ATCTGTGTTTCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCAGAATTTAAACGTTACA
ATGTGACGCCGTCTAACGTATATGAAATGTGGCAACAATTAGATACGCATAGCAGTAGTGCAGAGCAAAAGCTACTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGATTTCATTCAT
TTTCTCCTCAAGAGCTAGAAATTGTAAGAGGGCTAATGAGATTAGGAACTAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTGTTTTATGAAACGACGTTAACGTATGAAAGAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAATTTGAAAAAACAATTCCACTTATGGGACAGCCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAATGCATTATGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCGAGTGTGACAATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGAAGACTTGTGGCGGCTGAAGACTATCGTTACCGAGATATAGCAGT
ACTTCTTCGTAACGGAGAAAGTTATTATGATGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGCTAGTAGAATGTATTCGTTCGGCTTTAGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGTGTGAAAACAGAACTTTTATATCCACTAGACGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAAAGCGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTGTCTATGTTTACTGATGTAATGACGACAGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAATATTGATCATTCTAGATTATCAGATATTAAAGCGACGTTCATTATTGGCGTAAATGAAGGCG
TGATTCCCGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAGCTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGGGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAAACGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCA
GAAGTAACGTTGTCTTACGTCATGCAGCAACTGCAAACGTGGAAGCGTTACGGATTTGAAGGCAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTGTTATCGAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTCGCTCAGCACGGTTTATCGCTAAGAGAGCGCGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCATTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGCTTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCGCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAGGATGAGAGTGGT
ACGTTCCTTCGTATTATTGACTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGTAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGACGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTGTACGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCTTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
CCAATTCCGTATGTTAAAAGATATGAAAGATAGCGAAGCAATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.063

100

0.492