Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PLE7327_RS11130 Genome accession   NC_019689
Coordinates   2498094..2500115 (-) Length   673 a.a.
NCBI ID   WP_015143930.1    Uniprot ID   K9T405
Organism   Pleurocapsa sp. PCC 7327     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2493094..2505115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PLE7327_RS11115 (Ple7327_2316) - 2494973..2495548 (-) 576 WP_041392063.1 hypothetical protein -
  PLE7327_RS11120 (Ple7327_2317) - 2495664..2496884 (-) 1221 WP_015143928.1 type II secretion system F family protein -
  PLE7327_RS11125 (Ple7327_2318) pilT 2496921..2498030 (-) 1110 WP_015143929.1 type IV pilus twitching motility protein PilT Machinery gene
  PLE7327_RS11130 (Ple7327_2319) pilB/pilB1 2498094..2500115 (-) 2022 WP_015143930.1 GspE/PulE family protein Machinery gene
  PLE7327_RS11135 (Ple7327_2320) grpE 2500339..2501124 (+) 786 WP_015143931.1 nucleotide exchange factor GrpE -
  PLE7327_RS11140 (Ple7327_2321) dnaK 2501269..2503308 (+) 2040 WP_015143932.1 molecular chaperone DnaK -
  PLE7327_RS11145 (Ple7327_2322) dnaJ 2503455..2504582 (+) 1128 WP_015143933.1 molecular chaperone DnaJ -
  PLE7327_RS11150 (Ple7327_2323) - 2504579..2504821 (+) 243 WP_015143934.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75712.67 Da        Isoelectric Point: 5.4462

>NTDB_id=54765 PLE7327_RS11130 WP_015143930.1 2498094..2500115(-) (pilB/pilB1) [Pleurocapsa sp. PCC 7327]
MAYSSSSKRTRAVALRNYFSPFGNKLIQAGHATPEQMQQALIETRKSGRTLVEVLQSIVGRELPADLVRQYKKHHLFELK
ILYGVDCLDPELDPIANNEMADLIETLIAIDVCRRYKLVPLKKIDTQPPSVLVAMVDPDNLEAQDDLNRILRPRDIKLQR
TVITQEDYERLLEQYYEIQTEREKEKERLQKEKDLEKLADLSGIVENLDVNLQEAQDEINDDLGANEANQAPVINLVNKI
LAKALQENVSDIHVEPQEEYLRVRYRKDGVLQQAFDPLPKKITPAVVARFKIMAELDIAERRLPQDGKIRRMFQGRKVDF
RVNTLPSRYGEKVCLRILDNSATQLGLDKLITDQRTLDIVREMASRPFGLILVTGPTGSGKSTSLYSVLAERNHPGVNIS
TAEDPIEYSLPGITQVQVIREKGMDFANILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIAR
LDEMGVEPFMISGSLIGVLAQRLMRRVCTECRIPYHPTKEELARFGFSASNEGEVTFYKANTLTTEEIQEAKAKGTLCAK
CGGGGYKGRVGVYEVMQNSERLQSLINQGATTDRIKEAAVEEGMTTLLAYSLNLVREGYTTLEEVERVTFTDSGLEAELK
AKRKTALTCRTCHAELVPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=54765 PLE7327_RS11130 WP_015143930.1 2498094..2500115(-) (pilB/pilB1) [Pleurocapsa sp. PCC 7327]
ATGGCTTACTCTTCATCTTCAAAGCGGACTAGAGCCGTTGCTTTACGCAATTATTTTTCGCCTTTTGGGAACAAATTGAT
TCAAGCGGGTCATGCTACGCCCGAACAAATGCAGCAAGCATTAATCGAAACTCGTAAGTCCGGTCGTACTTTGGTAGAGG
TTCTCCAGTCGATCGTGGGTCGCGAACTCCCCGCCGATTTGGTTCGCCAGTATAAAAAACATCATTTATTTGAACTCAAA
ATTCTGTATGGAGTTGATTGCCTCGACCCAGAACTCGATCCCATCGCTAATAACGAGATGGCGGATCTCATCGAAACCTT
AATTGCGATCGATGTTTGTCGCCGTTATAAGCTAGTTCCTCTCAAAAAGATCGATACTCAGCCCCCTAGCGTTTTAGTAG
CGATGGTCGATCCGGATAACTTGGAAGCTCAAGACGACCTCAACCGTATCTTGCGCCCCAGAGACATCAAACTCCAGCGC
ACAGTCATTACCCAGGAAGATTACGAAAGATTGCTGGAACAATATTACGAGATACAGACTGAAAGAGAAAAAGAAAAAGA
GCGGCTTCAAAAAGAAAAAGACCTCGAAAAGTTAGCCGATCTTAGCGGGATCGTCGAAAATTTAGACGTTAACCTTCAAG
AAGCGCAAGACGAAATCAACGACGATCTGGGAGCTAACGAAGCCAATCAAGCCCCCGTCATCAATCTGGTCAACAAAATT
TTGGCAAAAGCCTTACAGGAAAATGTTTCTGACATTCACGTCGAGCCTCAAGAAGAATATCTGCGAGTGCGCTACCGCAA
AGATGGGGTGCTTCAGCAAGCCTTCGACCCCCTGCCCAAAAAGATTACGCCAGCCGTAGTCGCGCGTTTCAAAATTATGG
CAGAGTTGGACATTGCCGAGCGACGCTTGCCCCAAGACGGAAAAATTCGGCGGATGTTTCAAGGTCGCAAAGTGGACTTT
CGGGTTAATACCCTACCCAGCCGCTACGGAGAGAAAGTCTGTTTGCGGATTCTCGATAACTCGGCAACTCAGCTTGGTTT
GGATAAGTTGATTACCGACCAGAGAACCCTAGATATCGTTCGAGAAATGGCAAGCCGTCCCTTCGGATTGATCTTGGTAA
CGGGTCCGACGGGTTCCGGAAAGTCTACCTCGCTGTACTCGGTTCTAGCAGAACGAAACCATCCCGGCGTCAACATCAGT
ACGGCAGAAGACCCGATCGAATATTCGCTGCCAGGGATTACGCAGGTGCAGGTCATTCGCGAAAAAGGGATGGACTTTGC
CAATATTCTCAGGGCATTCATGCGGCAAGACCCAGACGTAATTCTAGTAGGTGAGACGCGAGATAAAGAAACGGCAAAAA
CGGCGATTGAAGCCGCACTGACGGGTCACTTGGTTTTGACTACCTTGCACACCAACGACGCGGCAGGCGCGATCGCCCGT
CTCGACGAAATGGGGGTCGAACCGTTTATGATTTCTGGCTCGTTAATCGGCGTTCTGGCTCAGCGTCTCATGCGCCGCGT
CTGTACCGAATGTCGCATTCCTTATCATCCAACCAAAGAAGAACTCGCTCGATTTGGCTTCTCGGCTTCTAACGAAGGAG
AAGTTACCTTCTACAAAGCTAACACCTTAACCACCGAAGAAATTCAAGAAGCCAAAGCGAAAGGCACTCTCTGCGCTAAG
TGTGGCGGTGGCGGTTACAAAGGACGGGTTGGCGTTTATGAGGTGATGCAAAACAGCGAGCGCCTGCAAAGCTTAATCAA
CCAAGGTGCAACCACCGACCGAATCAAAGAAGCCGCCGTCGAAGAGGGCATGACGACGCTGCTGGCTTACAGCTTAAATC
TGGTGCGAGAAGGCTATACAACCCTAGAAGAAGTAGAGCGAGTAACGTTTACCGATTCCGGTTTAGAGGCAGAACTCAAA
GCCAAACGCAAAACTGCTTTGACTTGTCGGACTTGTCATGCAGAACTGGTGCCGGAGTGGTTGGATTGTCCTTATTGCAT
GACCCCTCGCTTCAGCGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9T405

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

74.924

98.366

0.737
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.262

90.788

0.366

Multiple sequence alignment