Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NOS7524_RS22225 Genome accession   NC_019684
Coordinates   5465249..5467261 (-) Length   670 a.a.
NCBI ID   WP_015140725.1    Uniprot ID   K9QXH3
Organism   Nostoc sp. PCC 7524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5460249..5472261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOS7524_RS30495 - 5460642..5460878 (-) 237 WP_041555422.1 hypothetical protein -
  NOS7524_RS22205 (Nos7524_4563) - 5460924..5461928 (+) 1005 WP_015140721.1 saccharopine dehydrogenase-like oxidoreductase -
  NOS7524_RS22210 (Nos7524_4564) - 5461987..5462547 (-) 561 WP_015140722.1 hypothetical protein -
  NOS7524_RS22215 (Nos7524_4565) - 5462690..5463913 (-) 1224 WP_015140723.1 type II secretion system F family protein -
  NOS7524_RS22220 (Nos7524_4566) pilT 5464038..5465162 (-) 1125 WP_015140724.1 type IV pilus twitching motility protein PilT Machinery gene
  NOS7524_RS22225 (Nos7524_4567) pilB/pilB1 5465249..5467261 (-) 2013 WP_015140725.1 GspE/PulE family protein Machinery gene
  NOS7524_RS22230 (Nos7524_4568) grpE 5467625..5468371 (+) 747 WP_015140726.1 nucleotide exchange factor GrpE -
  NOS7524_RS22235 (Nos7524_4569) dnaK 5468625..5470586 (+) 1962 WP_015140727.1 molecular chaperone DnaK -
  NOS7524_RS22245 (Nos7524_4570) dnaJ 5471058..5472185 (+) 1128 WP_015140728.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75121.97 Da        Isoelectric Point: 5.1774

>NTDB_id=54748 NOS7524_RS22225 WP_015140725.1 5465249..5467261(-) (pilB/pilB1) [Nostoc sp. PCC 7524]
MTQSSPQRRSTALTTRTEFSPFGNKLVQSGYVNNDQMRQALIESRKSGRPLTEVLESITGRQLSPEFLRQYKKQQLFELK
ILYGVEFLDPEVNNLGNTMVGQLIETLIPVDICRRHRLVPLSRNDEQKPPYVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDELAVRQKHIEQEKFTDINQDLENLGNLDIQDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
ALHDKVSDIHVEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALAEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSDCRIPYTPTPEELARYGMSASQEVNVTFYKANTVPPEAIAETKAKNQLCPSCNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCNAVLQPEWLDCPYCMTPRFED

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=54748 NOS7524_RS22225 WP_015140725.1 5465249..5467261(-) (pilB/pilB1) [Nostoc sp. PCC 7524]
ATGACTCAATCTTCACCACAACGGCGCAGTACCGCACTCACTACAAGAACCGAGTTTTCGCCTTTTGGTAACAAGCTAGT
GCAATCTGGCTATGTAAATAACGACCAAATGAGGCAGGCGCTGATTGAAAGTCGCAAGTCTGGTAGACCCTTGACGGAAG
TGCTAGAGTCAATCACTGGACGACAATTATCACCTGAGTTTCTCAGGCAGTACAAGAAACAGCAATTATTTGAACTGAAG
ATACTATACGGTGTTGAATTCCTTGATCCGGAAGTCAATAATCTTGGCAATACGATGGTGGGGCAACTGATAGAAACCCT
CATCCCAGTGGATATCTGTCGCCGCCATCGTTTAGTCCCCCTATCTAGAAATGATGAACAAAAACCGCCCTATGTTTTAG
TGGCAATGGTTGATCCGGACAACTTAGAAGCGTCGGATGACCTCAACCGGATCTTGCGTCCCCAAGGTTTGGCATTACAG
CGCATGGTAATTACTCAGGAGGATTACCAACAGTTAATCAACCAATACTTGGATGAGTTGGCTGTCCGCCAAAAGCATAT
AGAACAAGAAAAATTTACAGATATTAATCAGGATTTAGAAAACCTCGGCAATCTCGATATTCAGGATGCGCCTGAAGAAA
TGGAGGCTGATTTAGGATCAGCAATGAAAGGTGCAGAGGATGCGCCAGTCATCAACCTTGTAAATCGCATCCTAGCTAAA
GCTCTGCACGATAAGGTTTCTGATATCCACGTCGAACCCCAAGAAGAAAATCTGCGGATTCGCTTTCGTAAGGATGGGGT
ATTACGGGAGGCTTTTGACCCCCTACCGAAAAAAATTATTCCCGCCGTCACAGCCAGATTTAAAATCATCTCTAACTTGG
ATATTGCTGAGAGACGTTTACCCCAAGACGGAAGGATTCGGCGGATGTTCGAGGGACGCAAAGTAGACTTCCGGGTGAAT
ACTTTGCCCAGTCGCTACGGGGAAAAGGTAGTGCTGCGGATTTTAGATAACTCTTCTACGCAATTAGGACTAAATAAGTT
AATTACCGACCCAGAAACCTTGCAAATTGTCCAAGATATGGTAAGTAAGCCTTTTGGGTTAATTTTGGTAACTGGGCCTA
CGGGTTCTGGTAAAACAACTTCACTATATTCAGCACTGGCAGAGAAGAACTCACCAGGGATTAATATCAGCACCGTAGAA
GATCCCATTGAGTACAGCTTGCCAGGGATTACACAAGTACAGGTAATTCGGGAAAAAGGTTTAGATTTTGCGACAGCTTT
GCGGGCATTTTTGCGACAAGATCCCGATGTGCTGCTAGTGGGTGAAACGCGGGATAAAGAAACAGCCAAAACAGCGATTG
AAGCTGCGTTGACTGGTCACTTAGTATTAACTACTTTACACACCAATGATGCTCCTGGTGCGATCGCACGTTTAGGAGAA
ATGGGTATTGAACCATTCATGGTTTCTAGTTCGTTGATTGGGGTATTGGCACAACGTCTAGTGCGGCGTGTTTGTTCTGA
TTGTCGCATTCCCTATACTCCCACACCTGAAGAATTAGCACGTTATGGGATGTCTGCTTCCCAAGAAGTCAACGTTACCT
TCTATAAAGCTAATACTGTACCACCAGAAGCGATCGCTGAAACCAAAGCCAAAAATCAGCTTTGTCCCAGCTGTAACGGT
GTCGGCTATAAAGGCCGTTGTGGTGTTTATGAAGTCATGCGGGTGACTGAAAACCTGCAAACTTTAATCAACGAAGAAGC
TCCCACAGAACGCATCAAAGAAGTAGCGGTAGAAGAAGGCATGAAAACCCTACTGGCGTACAGCTTAGACTTAGTTCGCC
AAGGTTCCACCACCCTAGAAGAAGTAGAACGGGTGACATTCACAGACACAGGTTTAGAAGCTGAATTAAAAGCCAAGCGT
AAAAGTGGACTTACCTGCCGCACCTGTAACGCCGTATTACAACCAGAATGGCTAGATTGTCCCTACTGTATGACACCTCG
GTTTGAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9QXH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.327

100

0.655
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.898

94.776

0.369

Multiple sequence alignment