Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LEPTO7376_RS14945 Genome accession   NC_019683
Coordinates   3319921..3321951 (+) Length   676 a.a.
NCBI ID   WP_015135001.1    Uniprot ID   K9Q0K3
Organism   [Leptolyngbya] sp. PCC 7376     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3314921..3326951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEPTO7376_RS14925 (Lepto7376_3005) - 3315152..3315412 (-) 261 WP_015134997.1 sulfurtransferase TusA family protein -
  LEPTO7376_RS14930 (Lepto7376_3006) dnaJ 3315412..3316551 (-) 1140 WP_015134998.1 molecular chaperone DnaJ -
  LEPTO7376_RS14935 (Lepto7376_3007) dnaK 3316670..3318625 (-) 1956 WP_015134999.1 molecular chaperone DnaK -
  LEPTO7376_RS14940 (Lepto7376_3008) grpE 3318768..3319511 (-) 744 WP_015135000.1 nucleotide exchange factor GrpE -
  LEPTO7376_RS14945 (Lepto7376_3009) pilB/pilB1 3319921..3321951 (+) 2031 WP_015135001.1 GspE/PulE family protein Machinery gene
  LEPTO7376_RS14950 (Lepto7376_3010) pilT 3322098..3323204 (+) 1107 WP_015135002.1 type IV pilus twitching motility protein PilT Machinery gene
  LEPTO7376_RS14955 (Lepto7376_3011) - 3323300..3324538 (+) 1239 WP_015135003.1 type II secretion system F family protein -
  LEPTO7376_RS14960 (Lepto7376_3012) - 3324602..3325183 (+) 582 WP_015135004.1 hypothetical protein -
  LEPTO7376_RS14965 (Lepto7376_3013) - 3325297..3326325 (+) 1029 WP_015135005.1 DUF928 domain-containing protein -

Sequence

Protein


Download         Length: 676 a.a.        Molecular weight: 75600.63 Da        Isoelectric Point: 4.9163

>NTDB_id=54728 LEPTO7376_RS14945 WP_015135001.1 3319921..3321951(+) (pilB/pilB1) [[Leptolyngbya] sp. PCC 7376]
MTQSPLSKRKKALALRNDFSPFGNKLIQSGVVDHQQMEKALVETRKSGRPLTSVIEELTGKALSPELVRQYKKHHLFELK
ILYGVDSIDPEIQDIDSSQMGELINSLIPIELCRRHKILPLRRIEIDEAIEVIVAMVDPDNLAAQDDINRLFRLQNIKMQ
RIVITDEDYRRILDEYLEVQVQQQADEKEEAERAAEADEMRKAVELDDLDLGDDEGLDEAADESADLMSENQASSAPVIK
LVNKVLLKALQEGVSDIHIEPQEKDLRIRFRKDGVLQYAFEPLPKKIIPAVSARFKIMAELDIAERRLPQDGRIRRVFKG
RKVDFRVNSLPSRYGEKICLRILDNSSTQLGLDILITNPDILEQVREMAQRPFGLILVTGPTGSGKSTTLYSILAERNHP
GVNISTAEDPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAP
RAIARLDEMGVEPFMISGSLLGVLAQRLMRRVCSECRVPYQPTADELGRFGLAASQDGELTIYKANTLSADQIQTARSKG
TLCPKCNGGGYKGRVGVYEFMRNTEEIEALINSGATTDLIKEVAVQEGMLSILAYSLDLVKEGYTTFDEVERVTFSDSGL
EAELKAKRKTGLTCGTCAAELKPEWVDCPYCMTPRF

Nucleotide


Download         Length: 2031 bp        

>NTDB_id=54728 LEPTO7376_RS14945 WP_015135001.1 3319921..3321951(+) (pilB/pilB1) [[Leptolyngbya] sp. PCC 7376]
ATGACTCAATCGCCTCTCTCAAAACGGAAGAAAGCCTTAGCGCTCCGTAATGATTTTTCTCCGTTTGGCAACAAACTCAT
CCAATCTGGCGTTGTTGACCACCAACAGATGGAAAAGGCTTTAGTTGAGACCCGTAAGAGCGGTCGACCCCTGACATCTG
TGATTGAGGAGCTTACTGGCAAGGCACTCTCCCCCGAATTAGTTCGACAATACAAAAAGCACCATCTTTTCGAACTTAAG
ATTCTTTATGGTGTTGATTCTATTGACCCTGAAATCCAGGATATCGATAGCAGTCAAATGGGAGAGCTGATCAATAGCCT
TATCCCTATTGAGCTTTGTCGCCGTCACAAAATTTTGCCTTTACGCCGTATTGAGATCGACGAAGCAATCGAAGTCATTG
TGGCAATGGTTGATCCGGATAATCTGGCTGCACAAGATGATATCAATCGTCTCTTCCGGCTGCAAAACATCAAAATGCAG
CGCATTGTAATTACAGATGAAGATTATCGGCGCATTCTCGACGAATATTTAGAAGTACAAGTTCAACAACAAGCCGACGA
GAAGGAAGAAGCAGAAAGAGCAGCCGAAGCAGACGAGATGCGTAAGGCTGTTGAGCTTGATGATCTTGACCTCGGTGATG
ATGAGGGACTAGATGAAGCTGCCGATGAAAGCGCTGATCTGATGAGTGAAAACCAGGCGAGTTCTGCGCCTGTGATTAAG
CTCGTTAACAAGGTTTTGCTCAAAGCTTTGCAGGAAGGAGTTTCTGATATTCACATAGAACCTCAAGAAAAAGATTTGCG
TATTCGATTCCGTAAGGATGGTGTATTGCAATATGCTTTTGAGCCCTTACCAAAGAAGATTATTCCGGCTGTATCTGCCC
GTTTCAAGATTATGGCAGAGCTTGATATTGCGGAGCGACGATTGCCGCAGGATGGTCGGATTCGTCGAGTCTTTAAAGGT
CGTAAAGTTGATTTTCGTGTGAACAGTCTTCCAAGTCGTTATGGCGAAAAGATTTGTCTCCGGATCTTGGATAACTCTTC
GACACAGCTAGGCTTAGATATTTTGATTACCAATCCAGATATTTTGGAGCAGGTGCGAGAAATGGCCCAGCGTCCATTTG
GTCTGATTTTGGTGACGGGACCCACGGGTTCTGGTAAATCGACGACACTGTATTCGATTCTCGCTGAACGAAATCATCCA
GGTGTCAACATTAGTACAGCGGAAGACCCGATTGAATATTCGCTTCCTGGTATCACGCAAGTTCAGGTAATTCGCGAAAA
AGGGATGGATTTTGCCTCGATTCTCCGCGCGTTTATGCGACAGGATCCAGATGTGATTCTGGTGGGTGAGACTCGCGATA
AAGAAACAGCAAAAACAGCGATTGAGGCAGCATTAACCGGTCACTTGGTTTTGACAACGCTTCATACGAACGACGCACCA
AGGGCGATCGCCCGATTGGATGAAATGGGTGTAGAGCCCTTTATGATTTCTGGATCATTGCTGGGTGTCCTAGCCCAACG
TCTTATGAGGCGGGTTTGTAGCGAATGTCGTGTGCCCTATCAACCGACCGCTGATGAACTAGGTCGCTTCGGTTTGGCAG
CTTCCCAAGATGGTGAACTTACAATTTATAAAGCCAATACTCTCAGTGCCGATCAAATCCAAACAGCACGCTCTAAAGGC
ACCCTATGTCCGAAGTGTAATGGTGGCGGCTATAAGGGTCGTGTTGGTGTGTATGAGTTTATGCGCAATACGGAGGAGAT
CGAAGCACTGATTAACAGCGGTGCCACTACTGATCTGATTAAAGAGGTTGCTGTGCAAGAAGGTATGCTCAGTATCTTGG
CCTACAGTCTTGATTTGGTAAAAGAGGGCTATACGACCTTTGATGAAGTCGAACGTGTAACCTTCTCCGATTCGGGCCTT
GAGGCAGAACTCAAAGCAAAACGGAAAACCGGGTTGACCTGTGGTACCTGTGCAGCTGAGCTAAAACCAGAATGGGTGGA
TTGCCCCTACTGTATGACGCCGCGTTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(244-511)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9Q0K3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

67.158

100

0.675

Multiple sequence alignment