Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   LRM32_RS00350 Genome accession   NZ_CP088131
Coordinates   73610..74278 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 81     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 68610..79278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRM32_RS00320 (LRM32_00320) yicC 68629..69492 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  LRM32_RS00325 (LRM32_00325) rph 69619..70335 (+) 717 WP_001247089.1 ribonuclease PH -
  LRM32_RS00330 (LRM32_00330) pyrE 70401..71042 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  LRM32_RS00335 (LRM32_00335) slmA 71079..71675 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  LRM32_RS00340 (LRM32_00340) dut 71782..72240 (-) 459 WP_000976070.1 dUTP diphosphatase -
  LRM32_RS00345 (LRM32_00345) coaBC 72218..73438 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  LRM32_RS00350 (LRM32_00350) radC 73610..74278 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  LRM32_RS00355 (LRM32_00355) rpmB 74495..74731 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  LRM32_RS00360 (LRM32_00360) rpmG 74752..74919 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  LRM32_RS00365 (LRM32_00365) mutM 75017..75826 (+) 810 WP_001114529.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  LRM32_RS00370 (LRM32_00370) coaD 75865..76344 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  LRM32_RS00375 (LRM32_00375) waaA 76352..77629 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  LRM32_RS00380 (LRM32_00380) rfaQ 78042..79100 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=547260 LRM32_RS00350 WP_001297375.1 73610..74278(+) (radC) [Escherichia coli strain 81]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=547260 LRM32_RS00350 WP_001297375.1 73610..74278(+) (radC) [Escherichia coli strain 81]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496