Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HRE53_RS01270 Genome accession   NZ_CP071717
Coordinates   266066..268072 (-) Length   668 a.a.
NCBI ID   WP_235108781.1    Uniprot ID   -
Organism   Acaryochloris sp. 'Moss Beach'     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261066..273072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRE53_RS01245 (HRE53_01210) - 261654..262407 (+) 754 Protein_228 isoprenyl transferase -
  HRE53_RS01250 (HRE53_01215) - 262470..262751 (-) 282 WP_235108779.1 DUF3143 domain-containing protein -
  HRE53_RS01255 (HRE53_01220) - 262942..263400 (-) 459 WP_235108780.1 J domain-containing protein -
  HRE53_RS01260 (HRE53_01225) - 263585..264805 (-) 1221 WP_212661864.1 type II secretion system F family protein -
  HRE53_RS01265 (HRE53_01230) pilT 264875..265996 (-) 1122 WP_212661865.1 type IV pilus twitching motility protein PilT Machinery gene
  HRE53_RS01270 (HRE53_01235) pilB/pilB1 266066..268072 (-) 2007 WP_235108781.1 GspE/PulE family protein Machinery gene
  HRE53_RS01275 (HRE53_01240) grpE 268692..269480 (+) 789 WP_212661869.1 nucleotide exchange factor GrpE -
  HRE53_RS01280 (HRE53_01245) dnaK 269644..271632 (+) 1989 WP_212661870.1 molecular chaperone DnaK -
  HRE53_RS01285 (HRE53_01250) dnaJ 271682..272806 (+) 1125 WP_212661871.1 molecular chaperone DnaJ -
  HRE53_RS01290 (HRE53_01255) - 272803..273063 (+) 261 WP_212661872.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74205.75 Da        Isoelectric Point: 4.7452

>NTDB_id=547251 HRE53_RS01270 WP_235108781.1 266066..268072(-) (pilB/pilB1) [Acaryochloris sp. 'Moss Beach']
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQSLPPELLRQYKRQQLFELKIL
YGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLTLDQETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRM
VIAVEDYQQLISKYLDQQAAQQKANEKPIEFDLDDDLDAIAGLDDLPTDELETDLGAALQDAETAPIIGLVNKILAKALQ
SGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISELDIAERRLPQDGRIRRLFQGRKIDFRVNTLP
SRYGEKIVLRILDNSSTQLGLDKLVADEDSLNIVREMVSRPFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPI
EYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPSPSELGRFGLSASPGTEITFYKANSLSADELQTAQATGQTVCSKCNGIG
YKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMKTLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKT
SLTCRVCNADLRPEWLDCPYCTTPRFQD

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=547251 HRE53_RS01270 WP_235108781.1 266066..268072(-) (pilB/pilB1) [Acaryochloris sp. 'Moss Beach']
ATGGTTAACTCTTCCTCACAGCGGCGTGCTCTAGTTACTCGGCACAATTTTTCCCCTTTTGGCAATAAGCTGATTTCAAC
AGGTTATGTTGACCAAGACCAAATGCGGGAAGCGACCTTAGAAAGTCGACGCTCCGGACGTTCTCTTGTCGAAATTCTTC
AGGGACTTACTGGACAGTCTTTACCACCAGAACTCCTACGCCAATATAAACGACAACAACTTTTTGAACTAAAGATCCTT
TATGGCATCGACTCCTTAGATCTTGAGTTAAGCCAAATTCCGACGGATCAAATCGAAGAATTGATCCAGAATTATATTTC
TCTTGACTTATGTCGACGCTATCAGTTGGTCCCCCTAACCCTTGATCAAGAAACGGACCCGGCAACGTTGCTGGTTGCAA
TGGTTGATCCAGATAATTTAGGTGCTCTGGACGATCTAGGACGAATTCTAAGGGAGAAAAATCTGACCTTGCAACGCATG
GTGATTGCGGTTGAAGACTATCAGCAACTCATCAGTAAGTATCTCGATCAGCAAGCTGCTCAGCAAAAAGCTAATGAAAA
ACCGATTGAGTTTGATTTAGACGATGACTTAGATGCGATCGCAGGTCTAGACGATCTCCCTACTGATGAACTTGAGACTG
ACCTCGGGGCAGCCCTGCAAGATGCAGAAACGGCTCCCATTATTGGTTTAGTTAACAAAATTCTGGCTAAAGCCTTGCAG
AGCGGTGTATCTGATATTCATATTGAACCGCAAGAAGATCACCTTCGTGTTCGATTCCGTAAAGATGGTGTGCTTCAACA
GGCCTTCGATCCACTGCCCAGGAAGATTATTCCTGCTGTTATATCCCGTTTCAAAATCATTTCAGAGTTAGATATTGCTG
AGCGTCGCCTTCCTCAAGATGGTCGCATCCGGCGACTTTTTCAGGGACGCAAGATTGACTTTCGGGTTAATACCTTACCG
AGCCGATATGGAGAGAAGATTGTACTTCGTATTCTGGATAACTCCTCGACTCAACTGGGATTAGACAAACTTGTTGCAGA
CGAAGATAGTTTAAACATCGTTAGAGAGATGGTCAGTAGACCTTTTGGCCTGATTTTAGTAACAGGACCTACTGGTTCTG
GTAAAACCACTACTCTCTATTCTGCCTTAGCTGAATGTAATCACCCGGGTGTCAACATCAGTACCGCTGAGGATCCAATT
GAATATACCCTCCCAGGCCTGACCCAAGTCCAGGTGATTCGAGAAAAAGGAATGGACTTTGCCTCAATTTTGAGAGCTTT
TCTAAGACAAGATCCAGATGTCATTCTAGTAGGTGAGACCCGAGATAAAGAAACTGCTAAAACAGCCATTGAAGCAGCAC
TAACAGGACACCTTGTGTTAACGACTCTGCATACGAATGATGCTGCTAGTGCTGTTGCTCGCCTTGCAGAAATGGAAGTC
GAGCCATTCATGGTTTCGGCGTCCTTATTGGGGGTTGTTGCCCAGCGTTTGGTACGGCGAGTATGTTCAGACTGTAAAGT
TCCCTATCAACCTTCTCCCTCTGAGCTAGGTCGATTTGGGCTATCTGCTTCTCCAGGAACAGAGATCACCTTCTACAAAG
CTAATTCGTTATCAGCTGATGAACTTCAAACAGCTCAAGCTACAGGACAAACAGTTTGTTCAAAATGTAACGGTATTGGG
TATAAAGGGCGTTGTGGTGTTTACGAAGTTATGCAAATCACTGAAACCCTGCAAACATTAATTACTGAAGGGGCACCTAC
TGAGCGGATTAAAGAGGTGGCAGTTGAAGAAGGGATGAAAACCCTTCTAGCTTATAGCCTAGAACTAGTTAAGAAAGGGG
ATACTACCCTCGCTGAAGTTGAACGAGTTACCTTTACCGATAGTGGTCTAGAGGCTGAACTTAAAGCTAAGCGTAAAACA
TCTCTAACTTGTCGAGTGTGTAATGCAGATTTAAGACCCGAGTGGTTAGATTGTCCCTATTGCACTACACCAAGATTCCA
AGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(233-500)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(58-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.404

99.251

0.639