Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NOS7107_RS06065 Genome accession   NC_019676
Coordinates   1410126..1412138 (-) Length   670 a.a.
NCBI ID   WP_015112100.1    Uniprot ID   K9Q9V3
Organism   Nostoc sp. PCC 7107     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1405126..1417138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOS7107_RS27150 (Nos7107_1215) - 1406537..1406776 (-) 240 WP_157373975.1 hypothetical protein -
  NOS7107_RS06050 (Nos7107_1216) - 1406990..1407547 (-) 558 WP_015112097.1 hypothetical protein -
  NOS7107_RS06055 (Nos7107_1217) - 1407642..1408865 (-) 1224 WP_015112098.1 type II secretion system F family protein -
  NOS7107_RS06060 (Nos7107_1218) pilT 1408969..1410087 (-) 1119 WP_015112099.1 type IV pilus twitching motility protein PilT Machinery gene
  NOS7107_RS06065 (Nos7107_1219) pilB/pilB1 1410126..1412138 (-) 2013 WP_015112100.1 GspE/PulE family protein Machinery gene
  NOS7107_RS06070 (Nos7107_1220) grpE 1412466..1413215 (+) 750 WP_015112101.1 nucleotide exchange factor GrpE -
  NOS7107_RS06080 (Nos7107_1221) dnaK 1413573..1415534 (+) 1962 WP_015112102.1 molecular chaperone DnaK -
  NOS7107_RS06085 (Nos7107_1222) dnaJ 1415986..1417116 (+) 1131 WP_015112103.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74645.30 Da        Isoelectric Point: 5.1120

>NTDB_id=54657 NOS7107_RS06065 WP_015112100.1 1410126..1412138(-) (pilB/pilB1) [Nostoc sp. PCC 7107]
MTQSSPQRRSTALTTRTEFSPFGSKLVQSGYVNSEQMRQALIESRKSGRPLTEVLESITGRQLSPEFLRQYKKQQLFELK
ILYGVESLDPEVNSIGNTNVGSLIETLIPVDICRRHRLVPLSKSEDQNPPSVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQFLDEMAVKQKHLEQEKFTDINQDLENLGNLDIQDAPDEMEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHVEPQEENLRIRFRKDGVLSEAFDPLPKKIIPAVTARFKIIASLDIAERRMPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLGIVQEMVSRPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSAAGEVELTFYKANTLTTEAVAEAKAKNHLCQTCNG
IGYKGRCGVYEVMRVTENLQTLINQEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVDRVTFTDTGLEAELRAKR
KSGLTCRTCSAELQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=54657 NOS7107_RS06065 WP_015112100.1 1410126..1412138(-) (pilB/pilB1) [Nostoc sp. PCC 7107]
ATGACTCAATCGTCACCACAACGGCGCAGTACCGCTCTCACGACCAGAACAGAGTTTTCGCCATTCGGCAGTAAGCTAGT
GCAATCTGGCTATGTTAATAGCGAACAGATGAGACAGGCACTGATTGAAAGTCGCAAATCTGGCAGGCCCCTGACGGAAG
TGCTGGAGTCGATCACTGGGCGACAACTATCGCCTGAATTTCTCAGGCAGTATAAAAAACAACAGTTATTTGAACTTAAA
ATACTATACGGCGTTGAATCTCTAGATCCGGAAGTCAACAGCATTGGTAATACAAATGTCGGCAGCCTGATTGAAACCTT
GATCCCGGTAGATATTTGTCGTCGCCATCGCTTAGTACCGCTATCTAAAAGTGAAGACCAAAACCCACCGAGCGTTTTAG
TGGCGATGGTCGATCCGGATAACTTGGAAGCTTCCGATGACCTAAATCGCATCTTGCGCCCTCAAGGCTTGGCATTGCAG
CGGATGGTGATCACTCAGGAAGATTATCAGCAACTCATCAACCAATTTTTAGATGAGATGGCTGTGAAGCAAAAGCATCT
GGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAATCTTGGTAATCTGGATATCCAAGATGCACCGGATGAAA
TGGAAGCTGATCTAGGATCTGCGATGAAAGGAGCAGAAGATGCTCCAGTAATCAACCTAGTTAATAGAATCTTGGCTAAG
GCTTTACACGAGAAGGTTTCGGATATTCACGTTGAACCTCAAGAAGAAAACTTACGCATTCGCTTTCGTAAGGATGGTGT
ATTGAGTGAAGCCTTCGATCCCTTACCGAAAAAAATTATTCCGGCGGTCACAGCCCGATTTAAAATTATCGCCAGCCTAG
ATATTGCCGAACGACGTATGCCTCAAGATGGACGCATACGTCGGATGTTTGAAGGTAGAAAAGTTGACTTTCGTGTGAAT
ACCTTACCCAGTCGCTATGGGGAAAAAGTAGTACTGCGGATTTTGGATAACTCATCTACTCAATTGGGATTGAATAAGTT
AATCACCGATCCAGAAACACTGGGTATTGTTCAAGAGATGGTTAGTCGTCCCTTTGGCCTAATTTTGGTAACTGGGCCGA
CAGGTTCTGGTAAAACAACTTCCCTGTATTCGGCATTATCAGAAAAGAACTCACCGGGAATTAATATCAGTACGGTTGAA
GACCCAATTGAGTATAGTTTGCCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAAGGACTGGATTTTGCCACAGCACT
GCGAGCCTTCTTGCGGCAAGACCCAGATGTGTTGCTAGTTGGTGAAACGCGGGATAAAGAAACCGCTAAAACAGCGATTG
AGGCTGCCTTAACAGGTCACTTAGTATTAACTACCTTACACACAAACGACGCACCAGGTGCGATCGCTCGTTTAGGTGAA
ATGGGCATTGAGCCATTCATGGTTTCTAGTTCTTTAATTGGTGTGTTGGCACAACGTTTAGTGCGACGCGTCTGTTCTGA
ATGCCGCATTCCTTACACTCCAACTACAGAAGAACTAGCTCGCTATGGTCTATCTGCTGCTGGTGAGGTCGAACTCACAT
TTTACAAAGCTAATACTTTAACCACAGAAGCCGTAGCAGAAGCCAAAGCTAAAAACCATCTTTGCCAAACATGTAATGGT
ATTGGGTACAAAGGACGCTGTGGTGTTTATGAAGTCATGCGCGTCACCGAAAATCTGCAAACCCTGATCAACCAAGAAGC
ACCGACAGAACGCATTAAAGAAGTTGCAGTAGAAGAAGGTATGAAAACCCTGTTGGCTTACAGCTTAGATTTAGTGCGTC
AAGGAGCTACCACCTTAGAAGAAGTTGATCGGGTGACATTTACCGATACAGGCTTAGAGGCCGAGTTAAGAGCCAAACGT
AAGAGTGGACTTACTTGTCGAACTTGCAGTGCCGAACTTCAACCAGAATGGCTGGATTGCCCCTACTGTATGACACCCAG
ATTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9Q9V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.667

100

0.669
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.725

95.97

0.372

Multiple sequence alignment