Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J1G53_RS02385 Genome accession   NZ_CP071490
Coordinates   482445..483026 (-) Length   193 a.a.
NCBI ID   WP_075592114.1    Uniprot ID   -
Organism   Glaesserella parasuis strain GHP1808     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477445..488026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G53_RS02345 (J1G53_02345) - 477792..478430 (+) 639 WP_015940081.1 nitroreductase family protein -
  J1G53_RS02350 (J1G53_02350) - 478519..479052 (+) 534 WP_207342528.1 TIGR00645 family protein -
  J1G53_RS02355 (J1G53_02355) - 479137..480435 (-) 1299 WP_075592116.1 NAD(P)/FAD-dependent oxidoreductase -
  J1G53_RS02375 (J1G53_02375) greB 480969..481448 (-) 480 WP_010786058.1 transcription elongation factor GreB -
  J1G53_RS02380 (J1G53_02380) - 481448..482383 (-) 936 WP_075592115.1 KpsF/GutQ family sugar-phosphate isomerase -
  J1G53_RS02385 (J1G53_02385) pilF 482445..483026 (-) 582 WP_075592114.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J1G53_RS02390 (J1G53_02390) - 483126..484286 (-) 1161 WP_035492316.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  J1G53_RS02395 (J1G53_02395) - 484487..484714 (-) 228 WP_016528332.1 hypothetical protein -
  J1G53_RS02400 (J1G53_02400) - 484820..485056 (-) 237 WP_015940086.1 hypothetical protein -
  J1G53_RS02425 (J1G53_02425) gltX 485890..487329 (+) 1440 WP_075592113.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22311.19 Da        Isoelectric Point: 8.0872

>NTDB_id=545636 J1G53_RS02385 WP_075592114.1 482445..483026(-) (pilF) [Glaesserella parasuis strain GHP1808]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSALAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=545636 J1G53_RS02385 WP_075592114.1 482445..483026(-) (pilF) [Glaesserella parasuis strain GHP1808]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACATGATGTAAAAGACTACCTACCGCACTCTGCGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

98.964

100

0.99
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.251

92.746

0.42