Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J1G47_RS09825 Genome accession   NZ_CP071487
Coordinates   1937647..1938228 (-) Length   193 a.a.
NCBI ID   WP_021110392.1    Uniprot ID   A0A084ELH5
Organism   Glaesserella parasuis strain YHP1818     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1932647..1943228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G47_RS09785 (J1G47_09785) - 1932995..1933633 (+) 639 WP_038514499.1 nitroreductase family protein -
  J1G47_RS09790 (J1G47_09790) - 1933722..1934255 (+) 534 WP_015940082.1 TIGR00645 family protein -
  J1G47_RS09795 (J1G47_09795) - 1934340..1935638 (-) 1299 WP_015940083.1 FAD-binding oxidoreductase -
  J1G47_RS09815 (J1G47_09815) greB 1936172..1936651 (-) 480 WP_010786058.1 transcription elongation factor GreB -
  J1G47_RS09820 (J1G47_09820) - 1936651..1937586 (-) 936 WP_026916781.1 KpsF/GutQ family sugar-phosphate isomerase -
  J1G47_RS09825 (J1G47_09825) pilF 1937647..1938228 (-) 582 WP_021110392.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J1G47_RS09830 (J1G47_09830) - 1938276..1939247 (-) 972 WP_012621529.1 IS110 family transposase -
  J1G47_RS09835 (J1G47_09835) - 1939490..1940650 (-) 1161 WP_035520425.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  J1G47_RS09840 (J1G47_09840) - 1940851..1941078 (-) 228 WP_016528332.1 hypothetical protein -
  J1G47_RS09845 (J1G47_09845) - 1941184..1941420 (-) 237 WP_015940086.1 hypothetical protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.24 Da        Isoelectric Point: 8.0872

>NTDB_id=545586 J1G47_RS09825 WP_021110392.1 1937647..1938228(-) (pilF) [Glaesserella parasuis strain YHP1818]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=545586 J1G47_RS09825 WP_021110392.1 1937647..1938228(-) (pilF) [Glaesserella parasuis strain YHP1818]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A084ELH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

99.482

100

0.995
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415