Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J0W32_RS10890 Genome accession   NZ_CP071317
Coordinates   2300181..2300978 (+) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain GXNN15X4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2295181..2305978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W32_RS10860 (J0W32_10860) - 2295498..2295917 (+) 420 WP_005182377.1 hypothetical protein -
  J0W32_RS10865 (J0W32_10865) - 2296085..2297368 (+) 1284 WP_206862650.1 glycosyltransferase family 4 protein -
  J0W32_RS10870 (J0W32_10870) - 2297401..2297952 (+) 552 WP_005182381.1 phosphatase PAP2 family protein -
  J0W32_RS10875 (J0W32_10875) iscX 2298047..2298247 (+) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  J0W32_RS10880 (J0W32_10880) ndk 2298356..2298787 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  J0W32_RS10885 (J0W32_10885) rlmN 2298910..2300145 (+) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  J0W32_RS10890 (J0W32_10890) pilF 2300181..2300978 (+) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J0W32_RS10895 (J0W32_10895) - 2300969..2301757 (+) 789 WP_016659532.1 helix-turn-helix domain-containing protein -
  J0W32_RS10900 (J0W32_10900) ispG 2301780..2302901 (+) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J0W32_RS10905 (J0W32_10905) hisS 2302905..2304200 (+) 1296 WP_005182398.1 histidine--tRNA ligase -
  J0W32_RS10910 (J0W32_10910) - 2304231..2304935 (+) 705 WP_005182401.1 YfgM family protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=544927 J0W32_RS10890 WP_104470591.1 2300181..2300978(+) (pilF) [Acinetobacter indicus strain GXNN15X4]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=544927 J0W32_RS10890 WP_104470591.1 2300181..2300978(+) (pilF) [Acinetobacter indicus strain GXNN15X4]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCACTGGAAACAGATTCGCGTGATTCTATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGTAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTCAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCTCTGCAGGCGAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCAGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCATTGTGGATTGGGATCCGTGTTGCACGAGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732