Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BN112_RS13665 Genome accession   NC_019382
Coordinates   2871971..2873011 (+) Length   346 a.a.
NCBI ID   WP_003808265.1    Uniprot ID   -
Organism   Bordetella bronchiseptica 253     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2866971..2878011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN112_RS13650 (BB253_2646) - 2867955..2868368 (-) 414 WP_015064516.1 DUF3592 domain-containing protein -
  BN112_RS13655 (BB253_2647) tssI 2868385..2870748 (-) 2364 WP_015064517.1 type VI secretion system tip protein VgrG -
  BN112_RS13660 (BB253_2648) - 2870897..2871736 (-) 840 WP_230594810.1 prepilin peptidase -
  BN112_RS13665 (BB253_2649) pilT 2871971..2873011 (+) 1041 WP_003808265.1 type IV pilus twitching motility protein PilT Machinery gene
  BN112_RS13670 (BB253_2650) - 2873046..2873840 (+) 795 WP_003808263.1 type II secretion system protein -
  BN112_RS13675 (BB253_2651) - 2873846..2874352 (+) 507 WP_003819516.1 hypothetical protein -
  BN112_RS13680 (BB253_2652) - 2874357..2875727 (+) 1371 WP_015064518.1 hypothetical protein -
  BN112_RS13685 (BB253_2653) - 2875724..2876233 (+) 510 WP_003808255.1 hypothetical protein -
  BN112_RS13690 (BB253_2654) - 2876259..2876858 (+) 600 WP_003808253.1 hypothetical protein -
  BN112_RS13695 (BB253_2655) - 2876855..2877625 (+) 771 WP_015064519.1 type II secretion system protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38021.71 Da        Isoelectric Point: 6.6652

>NTDB_id=54341 BN112_RS13665 WP_003808265.1 2871971..2873011(+) (pilT) [Bordetella bronchiseptica 253]
MTIDELLALTVARGASDLHLSAGLPPMLRINGDVVRQDVAALTPDALRAMIHQLMQPAQREEYETRQELDFSIDVEGLAR
FRVNAFNQQRGPAAVLRTIPSAIRTLDELGCPASFKELVRQPQGLILVTGPTGSGKSTTLAAMIDHLNREGEHHILTIED
PIEFVHPSRRSLVNQREVGRDTHSFSAALRSALREDPDIILVGELRDLETMRLAMTAAETGHLVFATLHTNSAAKTVDRI
IDVFPAGEKSMIRSMLSESLRAVIAQVLLKQEGGGRCAAWEIMVGTPAIRNLIREDKIAQMVSSIQTGQAHGMQTLDQAL
QDLLRKKLISAEQALAYARDRRSLGL

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=54341 BN112_RS13665 WP_003808265.1 2871971..2873011(+) (pilT) [Bordetella bronchiseptica 253]
ATGACCATCGATGAACTACTGGCCTTGACCGTGGCGCGCGGCGCCTCCGACCTGCATCTGTCGGCGGGGCTGCCGCCCAT
GCTGCGCATCAACGGCGACGTGGTGCGCCAGGACGTCGCGGCGCTCACGCCCGACGCCCTGCGCGCCATGATCCACCAGC
TGATGCAGCCGGCCCAGCGCGAAGAATACGAGACGCGCCAGGAGCTGGATTTCTCGATCGACGTCGAAGGCCTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGCAGCGCGGCCCGGCCGCGGTGCTGCGCACCATTCCGTCGGCCATCCGCACGCTGGA
CGAACTGGGCTGCCCGGCCTCGTTCAAGGAGCTGGTGCGCCAGCCGCAGGGGCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACCTCAACCGCGAAGGCGAGCATCACATCCTCACCATCGAGGAT
CCGATCGAGTTCGTGCACCCATCGCGCCGCAGCCTGGTCAACCAGCGCGAGGTCGGGCGCGACACCCACAGCTTCTCGGC
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATGCGCCTGG
CCATGACCGCCGCCGAGACCGGCCACCTGGTGTTCGCCACCTTGCACACCAACTCGGCCGCCAAGACGGTGGACCGCATC
ATCGATGTGTTTCCGGCCGGCGAGAAAAGCATGATCCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTCATCGCGCAAGT
CCTGCTCAAGCAGGAAGGCGGCGGACGCTGCGCCGCCTGGGAAATCATGGTCGGCACGCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGATCGCGCAGATGGTCTCCAGCATCCAGACCGGGCAGGCCCACGGCATGCAGACGCTGGACCAGGCGCTG
CAGGACCTGCTGCGCAAGAAACTGATTTCCGCCGAGCAGGCCCTGGCCTACGCCAGGGACCGGCGTTCGCTGGGCCTGTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

66.667

97.977

0.653
  pilT Pseudomonas aeruginosa PAK

66.372

97.977

0.65
  pilT Legionella pneumophila strain Lp02

66.766

96.532

0.645
  pilT Legionella pneumophila strain ERS1305867

66.766

96.532

0.645
  pilT Acinetobacter baylyi ADP1

63.953

99.422

0.636
  pilT Acinetobacter baumannii D1279779

62.791

99.422

0.624
  pilT Acinetobacter baumannii strain A118

62.791

99.422

0.624
  pilT Acinetobacter nosocomialis M2

62.791

99.422

0.624
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.063

96.243

0.607
  pilT Vibrio cholerae strain A1552

63.063

96.243

0.607
  pilT Neisseria meningitidis 8013

58.79

100

0.59
  pilT Neisseria gonorrhoeae MS11

58.501

100

0.587
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.146

99.133

0.497
  pilU Acinetobacter baylyi ADP1

42.529

100

0.428
  pilU Pseudomonas stutzeri DSM 10701

42.075

100

0.422
  pilU Vibrio cholerae strain A1552

42.183

97.977

0.413

Multiple sequence alignment