Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LGV82_RS01520 Genome accession   NZ_CP086104
Coordinates   313985..315610 (+) Length   541 a.a.
NCBI ID   WP_265069522.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RS650     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 308985..320610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGV82_RS01485 (LGV82_01480) - 309988..310554 (+) 567 WP_265069521.1 hypothetical protein -
  LGV82_RS01490 (LGV82_01485) - 310556..311044 (+) 489 WP_028854130.1 hypothetical protein -
  LGV82_RS01495 (LGV82_01490) - 311041..311517 (+) 477 WP_247393754.1 type II secretion system protein -
  LGV82_RS01500 (LGV82_01495) - 311511..311897 (+) 387 WP_247393758.1 type IV pilin protein -
  LGV82_RS01505 (LGV82_01500) - 311884..312348 (+) 465 WP_011003776.1 type II secretion system protein -
  LGV82_RS01510 (LGV82_01505) gspG 312352..312786 (+) 435 WP_011003775.1 type II secretion system major pseudopilin GspG -
  LGV82_RS01515 (LGV82_01510) - 312790..313983 (+) 1194 WP_247393761.1 type II secretion system F family protein -
  LGV82_RS01520 (LGV82_01515) pilF 313985..315610 (+) 1626 WP_265069522.1 GspE/PulE family protein Machinery gene
  LGV82_RS01525 (LGV82_01520) - 315603..316319 (+) 717 WP_247393765.1 hypothetical protein -
  LGV82_RS01530 (LGV82_01525) - 316309..316803 (+) 495 WP_247393768.1 hypothetical protein -

Sequence

Protein


Download         Length: 541 a.a.        Molecular weight: 59105.81 Da        Isoelectric Point: 6.3811

>NTDB_id=540340 LGV82_RS01520 WP_265069522.1 313985..315610(+) (pilF) [Ralstonia pseudosolanacearum strain RS650]
MSEWAPDWTAFGNWLRAARMARGSGSLATALAEQAGMPAQALVSAAAGRFGFHLLDAEGDLAPLVTLDPISLKDCRQHKI
LPLRIAGDWVIAMDDPWDEALVRWVNEKLGHARLAWMDGLHSLAILERALSTASASALLAQRGAEKTVQDQDVGRVVSHD
SIESTESAVVRFLDAVLLDAWRAGASDIHVETSRSGLHIKLRLDGVLVPGPAFTGARSPSEVLNRIKVLAQLDIGETRLP
QDGRLRVRLEGRDVDMRVSIMPNVFGEDAVIRLLDKAQLRGASETLTLEALGFDTAATATIRRLTKEPHGMLLVTGPTGS
GKTTTLYAALSEINTGRDKIITIEDPVEYELSGILQIPVNEKKGLTFARGLRSILRHDPDQILVGEIRDPETAEIAVQAA
LTGHLVFTSVHANSALDVVGRFLHMGVDPYNFMSALIGIVSQRLLRLKCPECAPASPDPGSRSVGCPACRYTGYRGRTVV
WEIVPVNDTIRELVIRREPLRLIREQSRQMGVVSLRDQAVRMVERGLTTFEEIDRVVSPNE

Nucleotide


Download         Length: 1626 bp        

>NTDB_id=540340 LGV82_RS01520 WP_265069522.1 313985..315610(+) (pilF) [Ralstonia pseudosolanacearum strain RS650]
ATGTCCGAATGGGCGCCCGATTGGACTGCGTTCGGCAACTGGCTGCGTGCCGCACGCATGGCGCGGGGCAGCGGCAGCCT
GGCCACGGCCCTCGCCGAGCAGGCCGGCATGCCGGCGCAGGCGCTGGTCTCGGCGGCGGCCGGCCGGTTCGGTTTCCATC
TGCTGGATGCGGAAGGCGACCTCGCCCCGCTGGTCACGCTCGACCCGATCTCGCTCAAGGACTGCCGCCAGCACAAGATC
CTGCCGCTGCGCATCGCCGGCGACTGGGTCATCGCGATGGACGACCCGTGGGACGAGGCGCTGGTGCGCTGGGTCAATGA
GAAGCTCGGCCATGCCAGGCTGGCCTGGATGGATGGTCTCCACAGTCTTGCCATCCTCGAACGCGCTCTGAGCACGGCTT
CGGCGTCCGCCCTGCTAGCCCAGCGAGGCGCGGAAAAGACGGTCCAGGACCAGGACGTCGGCCGTGTCGTCTCCCACGAC
TCCATCGAATCGACGGAAAGCGCCGTCGTACGCTTCCTCGACGCGGTATTGCTCGACGCCTGGCGGGCCGGTGCCAGCGA
CATCCACGTCGAGACCTCCCGCTCCGGCCTGCACATCAAGCTGCGGCTGGACGGCGTACTGGTGCCTGGCCCCGCGTTCA
CCGGTGCGCGCTCGCCCAGCGAAGTGCTGAACCGGATCAAAGTGCTGGCCCAGCTGGATATCGGCGAGACCCGGCTGCCG
CAGGACGGCCGCCTCCGCGTGCGACTGGAGGGGCGCGATGTCGACATGCGGGTCTCCATCATGCCCAACGTGTTCGGCGA
GGATGCCGTCATCCGCTTGCTGGACAAGGCGCAGTTGCGCGGCGCATCGGAAACGCTCACGCTCGAGGCGCTGGGCTTCG
ACACCGCGGCCACCGCCACCATCCGCCGGCTGACCAAGGAGCCGCACGGCATGCTGCTGGTGACCGGCCCCACCGGCAGC
GGCAAGACCACCACGCTGTACGCCGCGCTCTCTGAAATCAACACCGGGCGCGACAAGATCATCACGATCGAAGACCCGGT
CGAATACGAGCTGTCGGGCATCCTGCAGATTCCCGTCAACGAAAAGAAGGGCCTGACCTTTGCCCGCGGCCTGCGCTCGA
TCCTGCGCCACGACCCGGACCAGATCCTCGTCGGTGAAATCCGCGACCCCGAGACGGCGGAGATTGCTGTGCAAGCCGCG
CTGACCGGGCACTTGGTCTTCACGTCCGTGCACGCGAACTCGGCACTCGACGTGGTCGGCCGCTTCCTGCACATGGGGGT
CGACCCCTACAACTTCATGTCGGCGCTGATCGGCATCGTGTCGCAGCGGTTGCTGCGCCTGAAATGCCCGGAATGCGCGC
CTGCTTCTCCGGATCCGGGCAGCCGGTCCGTCGGATGCCCAGCCTGTCGGTACACCGGGTACCGAGGTCGTACCGTTGTC
TGGGAAATCGTCCCCGTCAATGACACGATCCGTGAGCTCGTGATCCGGCGCGAGCCGCTGCGGCTGATCCGCGAGCAGAG
CCGACAGATGGGCGTGGTGTCGTTGCGCGACCAGGCGGTGCGCATGGTGGAGCGTGGCCTGACGACGTTTGAGGAAATCG
ACCGCGTGGTGTCGCCCAATGAATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(176-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-111)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

36.581

100

0.368
  pilB Legionella pneumophila strain ERS1305867

35.832

100

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.567

99.076

0.362