Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   JQX68_RS05845 Genome accession   NZ_CP070483
Coordinates   1166116..1169820 (+) Length   1234 a.a.
NCBI ID   WP_147771724.1    Uniprot ID   -
Organism   Bacillus sp. LJBS06     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1161116..1174820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQX68_RS05830 (JQX68_05830) - 1161560..1161889 (-) 330 WP_014663456.1 four-helix bundle copper-binding protein -
  JQX68_RS05835 (JQX68_05835) - 1162008..1162445 (-) 438 WP_147771722.1 ferritin family protein -
  JQX68_RS05840 (JQX68_05840) addB 1162629..1166129 (+) 3501 WP_147771723.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  JQX68_RS05845 (JQX68_05845) addA 1166116..1169820 (+) 3705 WP_147771724.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  JQX68_RS05850 (JQX68_05850) sbcD 1169892..1171067 (+) 1176 WP_014663460.1 exonuclease subunit SbcD -
  JQX68_RS05855 (JQX68_05855) sbcC 1171064..1174456 (+) 3393 WP_014663461.1 exonuclease subunit SbcC -
  JQX68_RS05860 (JQX68_05860) hlpB 1174470..1174772 (+) 303 WP_014663462.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141305.60 Da        Isoelectric Point: 5.0880

>NTDB_id=539993 JQX68_RS05845 WP_147771724.1 1166116..1169820(+) (addA) [Bacillus sp. LJBS06]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEDLPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYRRVPAVAFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLTEMKPVIETLVQIVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAQNDKGEYEPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGKGTGRKIDLNKNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETDTELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAIEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRVFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGSALARHRDLEALAGNGTPVHADISGHPARFAVQMIHSYD
LLDDDVEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLNWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRALVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVQRLYEKELLTEEQKDAIDIEEIVQFFHTEIGG
QLIGAKWKDREIPFSLALPAKEIYPDAHEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYD
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=539993 JQX68_RS05845 WP_147771724.1 1166116..1169820(+) (addA) [Bacillus sp. LJBS06]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTTGTTGAACGAATGATTCGGAAAATCACCGCAGAGGAAAACC
CAATTGATGTAGACCGCCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGAATTGCGGATGCC
TTGGAAAAAGAGCTTGTACAACGCCCCGGCTCGCTGCATATCAGACGGCAGCTTTCTCTTTTGAACCGGGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTTGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCA
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTTAAACAAGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGAGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGACCTGCCGTTTTATCAATATGTCAAAGAGGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTAGCGCAGATTGATGAACT
GATTCAGCACCAGGACGATTTTAGTGAACTATATCGTCGTGTGCCAGCCGTCGCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCGGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCAAAAAAACTGCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGTCCTGAACAGCACTTGAAAAGCCTAACCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GATTGTTATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTCAGAATGACAAAGGTGAATATGAGCCGAGCGAGGCTGCAAGGTTTTATCAAGAACAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTACAGGAATCGATTCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACTGGCAACCTGTTTATGGTAGGCGATGTCAAACAGTCAATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGGAAAGGCACGGGGCGGAAAATCGACTTGAATAAAAATTTCCGAAGC
CGTTCAGATATATTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGACGAGACGGATACGGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACCGTGCAGTTTGAGGCCAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACATCGCAATATTCAATACCGAGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGTTATTTCGAAGCGATCGAAGTCGCCGTCGCGCTTTCAGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCTTACTATGAAGCGATGAAAGACTACTTGGCTGCTGGTGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTACGGACATCTGCAAAAATGGCGCGTCTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGACTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGCCAATATGAATCAACGGCATTTCGCGGCTTGTTTCGTTTCCTGCGGTTTATCGAACGCATGCAAGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGCCGGAATTTTAACATGATGGATTTAAATAAATCGTACCTGCTGGACAAGGA
GCTCGGATTCGGCACGAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCTCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGAATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAAACTGATTGGCTCCTTCCGGA
ATTTGACCGCTATCAGGCGAAAACATATTTAGATTTCATCGGATCGGCTCTTGCCAGACATAGAGACTTGGAGGCTTTGG
CTGGAAACGGTACACCAGTACACGCTGATATCTCAGGCCATCCAGCTCGTTTTGCCGTTCAAATGATTCATTCCTATGAT
TTGCTTGATGATGATGTGGAAGAAAGAATGGAAGAAAAAAGTGAACGTCTGGAAGCGATCCGCCGAGGGGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGCGAGCAGCTGAACTGGACCTACCCGCATCAAGAGGTGACACAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCTTGTAAAACCGGCT
GACGGAAGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGAACTGCCATGCA
TACGGTGATGCAGCATATCCCGTTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCAAAGGCTTTATGAAA
AAGAGCTGCTTACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAGATCGGGGGA
CAGCTGATCGGTGCGAAATGGAAGGACCGGGAAATACCTTTCAGCTTGGCGCTTCCGGCTAAGGAGATCTATCCTGATGC
ACACGAGGCGGATGAGCCGCTTTTAGTGCAGGGGATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATTTATTGG
ATTATAAGTCGGACCGGATTGAGGGCAAATTCCAACATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAT
ACCCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTCACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

97.326

100

0.973