Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JTF64_RS25780 Genome accession   NZ_CP070339
Coordinates   5089319..5090362 (+) Length   347 a.a.
NCBI ID   WP_001013181.1    Uniprot ID   -
Organism   Bacillus cereus strain VKM B-370     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5084319..5095362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTF64_RS25745 (JTF64_25700) - 5084684..5085172 (+) 489 WP_000764263.1 hypothetical protein -
  JTF64_RS25750 (JTF64_25705) - 5085207..5085518 (-) 312 WP_001093240.1 hypothetical protein -
  JTF64_RS25755 (JTF64_25710) - 5085772..5085987 (+) 216 WP_001008326.1 DUF3912 family protein -
  JTF64_RS25760 (JTF64_25715) - 5086075..5086467 (+) 393 WP_000877908.1 hypothetical protein -
  JTF64_RS25765 (JTF64_25720) - 5086547..5087941 (-) 1395 WP_001094344.1 L-cystine transporter -
  JTF64_RS25770 (JTF64_25725) - 5088049..5088291 (-) 243 WP_000375619.1 DUF2626 domain-containing protein -
  JTF64_RS25775 (JTF64_25730) - 5088418..5089113 (-) 696 WP_000434110.1 helix-turn-helix domain-containing protein -
  JTF64_RS25780 (JTF64_25735) comGA 5089319..5090362 (+) 1044 WP_001013181.1 competence type IV pilus ATPase ComGA Machinery gene
  JTF64_RS25785 (JTF64_25740) comGB 5090349..5091386 (+) 1038 WP_003257965.1 competence type IV pilus assembly protein ComGB -
  JTF64_RS25790 (JTF64_25745) comGC 5091398..5091697 (+) 300 WP_001178680.1 competence type IV pilus major pilin ComGC -
  JTF64_RS25795 (JTF64_25750) comGD 5091694..5092149 (+) 456 WP_002082168.1 competence type IV pilus minor pilin ComGD -
  JTF64_RS25800 (JTF64_25755) - 5092142..5092444 (+) 303 WP_000829465.1 hypothetical protein -
  JTF64_RS25805 (JTF64_25760) comGF 5092414..5092884 (+) 471 WP_000923110.1 competence type IV pilus minor pilin ComGF -
  JTF64_RS25810 (JTF64_25765) comGG 5092881..5093252 (+) 372 WP_001214886.1 competence type IV pilus minor pilin ComGG -
  JTF64_RS25815 (JTF64_25770) - 5093427..5094077 (+) 651 WP_000183866.1 2OG-Fe(II) oxygenase -
  JTF64_RS25820 (JTF64_25775) - 5094193..5094690 (+) 498 WP_000836666.1 shikimate kinase -
  JTF64_RS25825 (JTF64_25780) - 5094729..5094929 (+) 201 WP_000106083.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39168.55 Da        Isoelectric Point: 8.7212

>NTDB_id=539240 JTF64_RS25780 WP_001013181.1 5089319..5090362(+) (comGA) [Bacillus cereus strain VKM B-370]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEANGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=539240 JTF64_RS25780 WP_001013181.1 5089319..5090362(+) (comGA) [Bacillus cereus strain VKM B-370]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGATATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGTATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTTACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAATGTCCGTTTTGCAGAGGGAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGGTATGAGTTAAAACAAGCGCTTAAAGAAGCGAATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGGTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369