Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   LIN94_RS00360 Genome accession   NZ_CP085092
Coordinates   73929..74480 (+) Length   183 a.a.
NCBI ID   WP_025298068.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 30R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 68929..79480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN94_RS00345 - 69879..71243 (+) 1365 WP_005547462.1 L-serine ammonia-lyase -
  LIN94_RS00350 ansB 71424..72473 (-) 1050 WP_005568022.1 L-asparaginase 2 -
  LIN94_RS00355 - 72768..73907 (+) 1140 WP_025298067.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  LIN94_RS00360 pilF/pilF2 73929..74480 (+) 552 WP_025298068.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LIN94_RS00365 - 74624..75679 (+) 1056 WP_005568019.1 RodZ family helix-turn-helix domain-containing protein -
  LIN94_RS00370 ispG 75691..76794 (+) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LIN94_RS00375 hisS 76816..78093 (+) 1278 WP_005589329.1 histidine--tRNA ligase -
  LIN94_RS00380 - 78104..78718 (+) 615 WP_025298069.1 YfgM family protein -
  LIN94_RS00385 - 78771..79223 (+) 453 WP_005568016.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20697.48 Da        Isoelectric Point: 8.8869

>NTDB_id=537601 LIN94_RS00360 WP_025298068.1 73929..74480(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 30R]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKAFSYAPDYYLVSAAFAYF
YQQQGDIEQARTTYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPKRAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=537601 LIN94_RS00360 WP_025298068.1 73929..74480(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 30R]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTACCACCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGAAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.576

90.164

0.519
  pilF Glaesserella parasuis strain SC1401

46.471

92.896

0.432