Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LH370_RS11125 Genome accession   NZ_CP085063
Coordinates   2284820..2285260 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain P31_YH1_02.21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2279820..2290260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LH370_RS11105 (LH370_11105) aroP 2280818..2282191 (+) 1374 WP_001347572.1 aromatic amino acid transporter AroP -
  LH370_RS11110 (LH370_11110) ampE 2282234..2283088 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  LH370_RS11115 (LH370_11115) ampD 2283085..2283636 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LH370_RS11120 (LH370_11120) nadC 2283724..2284617 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LH370_RS11125 (LH370_11125) pilA 2284820..2285260 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  LH370_RS11130 (LH370_11130) pilB 2285270..2286655 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  LH370_RS11135 (LH370_11135) hofC 2286645..2287847 (+) 1203 WP_187791211.1 protein transport protein HofC -
  LH370_RS11140 (LH370_11140) guaC 2287882..2288925 (-) 1044 WP_001217338.1 GMP reductase -
  LH370_RS11145 (LH370_11145) - 2289081..2289125 (-) 45 WP_120795372.1 protein YacM -
  LH370_RS11150 (LH370_11150) coaE 2289150..2289770 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=536742 LH370_RS11125 WP_000360895.1 2284820..2285260(+) (pilA) [Escherichia coli strain P31_YH1_02.21]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=536742 LH370_RS11125 WP_000360895.1 2284820..2285260(+) (pilA) [Escherichia coli strain P31_YH1_02.21]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363