Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D1099_RS07360 Genome accession   NZ_CP069796
Coordinates   1566608..1567156 (-) Length   182 a.a.
NCBI ID   WP_005608494.1    Uniprot ID   A0A828PJH3
Organism   Actinobacillus pleuropneumoniae serovar 6 str. Femo     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1561608..1572156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1099_RS07345 (D1099_07325) - 1562090..1562611 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  D1099_RS07350 (D1099_07330) - 1562716..1564002 (+) 1287 WP_005608492.1 NAD(P)/FAD-dependent oxidoreductase -
  D1099_RS07355 (D1099_07335) - 1564194..1566533 (-) 2340 WP_005608493.1 Tex family protein -
  D1099_RS07360 (D1099_07340) pilF 1566608..1567156 (-) 549 WP_005608494.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  D1099_RS07365 (D1099_07345) - 1567210..1568391 (-) 1182 WP_005608495.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  D1099_RS07390 (D1099_07370) gltX 1569297..1570736 (+) 1440 WP_005608501.1 glutamate--tRNA ligase -
  D1099_RS07400 (D1099_07380) yefM 1571074..1571325 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  D1099_RS07405 (D1099_07385) - 1571322..1571582 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  D1099_RS07410 (D1099_07390) - 1571741..1571908 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20596.33 Da        Isoelectric Point: 7.7373

>NTDB_id=536693 D1099_RS07360 WP_005608494.1 1566608..1567156(-) (pilF) [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNGYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSNNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=536693 D1099_RS07360 WP_005608494.1 1566608..1567156(-) (pilF) [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
ATGAATCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACGTTTAATCGTTCGGAAGCGGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGGTTACCTACCTCACTCCGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAGCTGAGTAATAACCGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATCGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAAGCAAGAGCGGCGGCACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A828PJH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

67.021

100

0.692
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44