Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JS559_RS07160 Genome accession   NZ_CP069795
Coordinates   1541575..1542123 (-) Length   182 a.a.
NCBI ID   WP_011848557.1    Uniprot ID   A3N1S3
Organism   Actinobacillus pleuropneumoniae strain A-85/14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1536575..1547123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JS559_RS07145 (JS559_07145) - 1537057..1537578 (+) 522 WP_193327846.1 SprT family zinc-dependent metalloprotease -
  JS559_RS07150 (JS559_07150) - 1537683..1538969 (+) 1287 WP_005615681.1 NAD(P)/FAD-dependent oxidoreductase -
  JS559_RS07155 (JS559_07155) - 1539161..1541500 (-) 2340 WP_011848556.1 Tex family protein -
  JS559_RS07160 (JS559_07160) pilF 1541575..1542123 (-) 549 WP_011848557.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  JS559_RS07165 (JS559_07165) - 1542177..1543358 (-) 1182 WP_009875290.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  JS559_RS07190 (JS559_07190) gltX 1544262..1545701 (+) 1440 WP_005605060.1 glutamate--tRNA ligase -
  JS559_RS07200 (JS559_07200) yefM 1546039..1546290 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  JS559_RS07205 (JS559_07205) - 1546287..1546547 (+) 261 WP_237611416.1 Txe/YoeB family addiction module toxin -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20671.35 Da        Isoelectric Point: 6.9479

>NTDB_id=536665 JS559_RS07160 WP_011848557.1 1541575..1542123(-) (pilF) [Actinobacillus pleuropneumoniae strain A-85/14]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=536665 JS559_RS07160 WP_011848557.1 1541575..1542123(-) (pilF) [Actinobacillus pleuropneumoniae strain A-85/14]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCCGTGTTATTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3N1S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

67.021

100

0.692
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44