Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LH706_RS02295 Genome accession   NZ_CP085043
Coordinates   507879..509435 (-) Length   518 a.a.
NCBI ID   WP_028853770.1    Uniprot ID   A0ABX7ZQ67
Organism   Ralstonia pseudosolanacearum strain RS638     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 502879..514435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LH706_RS02280 (LH706_02295) - 503713..504999 (-) 1287 WP_020371584.1 hypothetical protein -
  LH706_RS02285 (LH706_02300) - 505075..506358 (-) 1284 WP_020832936.1 hypothetical protein -
  LH706_RS02290 (LH706_02305) gspF 506666..507877 (-) 1212 WP_021154964.1 type II secretion system inner membrane protein GspF -
  LH706_RS02295 (LH706_02310) pilF 507879..509435 (-) 1557 WP_028853770.1 type II secretion system ATPase GspE Machinery gene
  LH706_RS02300 (LH706_02315) gspD 509470..511887 (-) 2418 WP_020832934.1 type II secretion system secretin GspD -
  LH706_RS02305 (LH706_02320) - 511921..512772 (-) 852 WP_058908063.1 type II secretion system protein N -
  LH706_RS02310 (LH706_02325) gspM 512776..513354 (-) 579 WP_011003003.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55861.86 Da        Isoelectric Point: 5.5004

>NTDB_id=536497 LH706_RS02295 WP_028853770.1 507879..509435(-) (pilF) [Ralstonia pseudosolanacearum strain RS638]
MATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFGALRLVSLDAAALSAA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVTPEIQTMIHRQAPES
EIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=536497 LH706_RS02295 WP_028853770.1 507879..509435(-) (pilF) [Ralstonia pseudosolanacearum strain RS638]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGCCGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTATGT
GTTCGCGCGCGACGCCAGGCTGCTGGTGGCGCGGCAGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAGCTGGCCCGCGTGTTCGGCGCGCTGCGCCTGGTGAGCCTGGACGCCGCCGCGCTCTCCGCCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTCGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGACCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCACGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGCTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGACCGGTGGACGTGC
GTGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTGCTGCGCCTGCTCGACAAGGAAGCCGGCCGGCTCGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACGCTGGGGCGCTTCGACCGCCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACCGGCTCGGGCAAGACCACCACGCTGTATGCCGCGCTGTCGCGGCTCGATGCCAGCAGCACCAACATCATGA
CGGTGGAGGACCCGATCGAGTACGACCTGGACGGCATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGGCAGGACCCGGACGTGGTGATGATCGGCGAGATCCGCGACCTGGAGACCGCGCAGAT
CGCGGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAGCTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCCGA
CGGCACCGCCGCCGCCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAGCACTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGCTGCTGACGGTGACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGCCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCATACCATGCGCGCGGATGCGCAGCGGTGGCTCGACGCGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

42.744

97.104

0.415
  pilB Vibrio cholerae strain A1552

45.657

86.68

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.689

99.228

0.394
  pilB Vibrio campbellii strain DS40M4

44.835

87.838

0.394
  pilB/pilB1 Synechocystis sp. PCC 6803

44.676

83.398

0.373
  pilB Vibrio parahaemolyticus RIMD 2210633

46.324

78.764

0.365