Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I6J37_RS10900 Genome accession   NZ_CP069486
Coordinates   2153313..2154215 (+) Length   300 a.a.
NCBI ID   WP_103323129.1    Uniprot ID   -
Organism   Mammaliicoccus vitulinus strain FDAARGOS_1207     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2148313..2159215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J37_RS10860 (I6J37_10860) - 2148396..2148950 (+) 555 WP_103323127.1 5-formyltetrahydrofolate cyclo-ligase -
  I6J37_RS10865 (I6J37_10865) - 2148951..2150399 (+) 1449 WP_103323128.1 rhomboid family intramembrane serine protease -
  I6J37_RS10870 (I6J37_10870) - 2150403..2150594 (+) 192 WP_016911882.1 YqgQ family protein -
  I6J37_RS10875 (I6J37_10875) - 2150591..2151562 (+) 972 WP_016911883.1 ROK family glucokinase -
  I6J37_RS10880 (I6J37_10880) - 2151562..2151876 (+) 315 WP_016911884.1 MTH1187 family thiamine-binding protein -
  I6J37_RS10885 (I6J37_10885) - 2151906..2152181 (-) 276 WP_224658742.1 DUF2759 domain-containing protein -
  I6J37_RS10890 (I6J37_10890) - 2152245..2152877 (+) 633 WP_016911886.1 MBL fold metallo-hydrolase -
  I6J37_RS10895 (I6J37_10895) - 2152914..2153159 (-) 246 WP_016911887.1 DUF2626 family protein -
  I6J37_RS10900 (I6J37_10900) comGA 2153313..2154215 (+) 903 WP_103323129.1 competence type IV pilus ATPase ComGA Machinery gene
  I6J37_RS10905 (I6J37_10905) comGB 2154208..2155245 (+) 1038 WP_204107810.1 competence type IV pilus assembly protein ComGB -
  I6J37_RS10910 (I6J37_10910) comGC 2155259..2155576 (+) 318 WP_016911890.1 competence type IV pilus major pilin ComGC Machinery gene
  I6J37_RS10915 (I6J37_10915) comGD 2155576..2156004 (+) 429 WP_103323612.1 competence type IV pilus minor pilin ComGD -
  I6J37_RS10920 (I6J37_10920) comGE 2156027..2156269 (+) 243 WP_226908889.1 competence type IV pilus minor pilin ComGE -
  I6J37_RS10925 (I6J37_10925) comGF 2156244..2156678 (+) 435 WP_204107811.1 competence type IV pilus minor pilin ComGF -
  I6J37_RS10930 (I6J37_10930) - 2156913..2157401 (+) 489 WP_103323613.1 shikimate kinase -
  I6J37_RS10935 (I6J37_10935) gcvT 2157594..2158682 (+) 1089 WP_103323614.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34665.86 Da        Isoelectric Point: 6.4127

>NTDB_id=535032 I6J37_RS10900 WP_103323129.1 2153313..2154215(+) (comGA) [Mammaliicoccus vitulinus strain FDAARGOS_1207]
MEKLFKSIIEDAIKSNVSDIHFIPVIDDKIVVQMRFNGDNKDYDLSLDAKLYNRLLIYMKFISHLDVSERNKAQSGQYIY
EHVMNYYLRISTIPISLGIESCAIRITPQYFIESDANRLNSDFIEIRNKMYQSEGLFLFTGPTGSGKTTLMYELMYDLKK
EKNKHIITIEDPVEQTLNGVVQVSVNERANISYQQSFKAILRCDPDVIMIGEIRDEETAKQVIQASLSGHLVLSTMHAKD
NIGAIHRLIELGITVEQIKQGISCIANQRLVKQDESSRCRKMSYMFSEHIIQYMVRDYHE

Nucleotide


Download         Length: 903 bp        

>NTDB_id=535032 I6J37_RS10900 WP_103323129.1 2153313..2154215(+) (comGA) [Mammaliicoccus vitulinus strain FDAARGOS_1207]
ATGGAGAAATTATTTAAATCAATTATCGAAGACGCTATAAAATCAAATGTAAGCGATATTCATTTCATACCTGTAATAGA
CGATAAGATTGTTGTTCAGATGAGATTTAATGGTGACAACAAAGACTATGATTTATCGCTAGATGCTAAATTGTATAACA
GATTGCTAATCTATATGAAATTTATTTCTCACTTAGACGTAAGTGAGAGAAACAAAGCACAAAGTGGTCAGTATATTTAT
GAACATGTTATGAATTATTATTTAAGAATTTCGACGATACCAATTAGTTTAGGGATAGAATCATGTGCAATCAGGATAAC
ACCTCAATATTTTATTGAAAGTGATGCAAATAGGCTTAATTCAGATTTTATAGAAATTAGAAATAAGATGTATCAATCTG
AAGGTTTATTTCTATTTACTGGGCCTACAGGATCTGGTAAGACAACTTTAATGTATGAATTAATGTATGACTTAAAGAAA
GAAAAGAATAAACATATTATTACGATTGAGGATCCTGTGGAACAAACATTAAATGGCGTTGTACAAGTTTCTGTTAATGA
AAGAGCGAATATATCTTATCAACAGTCATTTAAAGCAATTTTAAGATGTGATCCTGATGTTATTATGATAGGTGAAATAA
GAGATGAAGAAACTGCTAAGCAAGTTATACAAGCAAGTTTAAGTGGGCATCTTGTGCTTTCAACTATGCATGCTAAAGAT
AATATCGGTGCAATACATAGATTAATTGAATTAGGCATAACTGTAGAACAAATTAAGCAAGGCATATCGTGTATTGCAAA
TCAGCGATTAGTTAAGCAAGATGAATCAAGTCGCTGTCGAAAAATGAGTTACATGTTTAGTGAACATATCATTCAATATA
TGGTGCGTGATTATCATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.832

99

0.493
  comGA Staphylococcus aureus N315

49.832

99

0.493
  comGA Bacillus subtilis subsp. subtilis str. 168

42.238

92.333

0.39