Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JR311_RS02920 Genome accession   NZ_CP069391
Coordinates   555857..556294 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Sam8H1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 550857..561294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JR311_RS20640 - 551454..551582 (-) 129 Protein_533 hypothetical protein -
  JR311_RS02885 (JR311_02885) - 551727..552929 (+) 1203 WP_204530260.1 GTP-binding protein -
  JR311_RS02890 (JR311_02890) - 552994..554130 (+) 1137 WP_204530261.1 zinc-dependent alcohol dehydrogenase -
  JR311_RS02895 (JR311_02895) - 554145..554579 (+) 435 WP_003156592.1 RDD family protein -
  JR311_RS02900 (JR311_02900) - 554696..554971 (+) 276 WP_369719109.1 YckD family protein -
  JR311_RS20910 - 555110..555180 (+) 71 Protein_538 family 1 glycosylhydrolase -
  JR311_RS02910 (JR311_02910) - 555187..555416 (+) 230 Protein_539 family 1 glycosylhydrolase -
  JR311_RS02915 (JR311_02915) nin/comJ 555435..555833 (-) 399 WP_204530263.1 competence protein ComJ Regulator
  JR311_RS02920 (JR311_02920) nucA/comI 555857..556294 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  JR311_RS02925 (JR311_02925) hxlB 556653..557210 (-) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  JR311_RS02930 (JR311_02930) hxlA 557207..557842 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  JR311_RS02935 (JR311_02935) - 558074..558436 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=534346 JR311_RS02920 WP_003156588.1 555857..556294(-) (nucA/comI) [Bacillus velezensis strain Sam8H1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=534346 JR311_RS02920 WP_003156588.1 555857..556294(-) (nucA/comI) [Bacillus velezensis strain Sam8H1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTCACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641