Detailed information NTG0533487

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: JQN63_RS06530
• Genome accession: NZ_CP069318
• Coordinates: 1430960..1431853 (-)
• Length: 297 a.a.
• NCBI ID: WP_016445609.1
• Uniprot ID: A0A1H3L211
• Organism: Delftia lacustris strain R54
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1425960..1436853

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQN63_RS06500 (JQN63_06505)	argJ	1426286..1427518 (+)	1233	WP_203400445.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
JQN63_RS06505 (JQN63_06510)	-	1427684..1428640 (+)	957	WP_126310904.1	ATP-binding protein	-
JQN63_RS06510 (JQN63_06515)	mutT	1428691..1429197 (+)	507	WP_043789746.1	8-oxo-dGTP diphosphatase MutT	-
JQN63_RS06515 (JQN63_06520)	-	1429208..1429462 (-)	255	WP_016451023.1	DNA gyrase inhibitor YacG	-
JQN63_RS06520 (JQN63_06525)	zapD	1429462..1430217 (-)	756	WP_016445607.1	cell division protein ZapD	-
JQN63_RS06525 (JQN63_06530)	coaE	1430307..1430945 (-)	639	WP_016445608.1	dephospho-CoA kinase	-
JQN63_RS06530 (JQN63_06535)	pilD	1430960..1431853 (-)	894	WP_016445609.1	prepilin peptidase	Machinery gene
JQN63_RS06535 (JQN63_06540)	pilC	1431853..1433070 (-)	1218	WP_047326532.1	type II secretion system F family protein	Machinery gene
JQN63_RS06540 (JQN63_06545)	pilB	1433109..1434851 (-)	1743	WP_203400446.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JQN63_RS06550 (JQN63_06555)	-	1435088..1436017 (-)	930	WP_043789750.1	polyprenyl synthetase family protein	-
JQN63_RS06555 (JQN63_06560)	rplU	1436248..1436559 (+)	312	WP_012207284.1	50S ribosomal protein L21	-
JQN63_RS06560 (JQN63_06565)	rpmA	1436575..1436832 (+)	258	WP_012207283.1	50S ribosomal protein L27	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 31842.37 Da
• Isoelectric Point: 6.9135

>NTDB_id=533487 JQN63_RS06530 WP_016445609.1 1430960..1431853(-) (pilD) [Delftia lacustris strain R54]
MVLELWMQAALGGLLGLLVGSFLNVVVHRLPRMMEQQWNAECAQWAQEQSSSSATPSPATAPPPAEAPLSLSRPRSRCPH
CGHAIHWYENIPVLSYLALRGRCSECKASISIRYPLVELACAALFAYCAAQWGITPTGAAWCLFSAALLSLALIDWDTTL
LPDDITLPLLWAGLLSSALHWTAVPLADAVWGAAAGYLSLWLVYWAFKLATGKEGMGYGDFKLFAALGAWFGWQALVPII
LISSVIGAIVGIALKINSRLREGGYVPFGPFLAGAGFAAMVFGPQQMLQSLLAALGL

Nucleotide

• Length: 894 bp

>NTDB_id=533487 JQN63_RS06530 WP_016445609.1 1430960..1431853(-) (pilD) [Delftia lacustris strain R54]
ATGGTTTTGGAACTCTGGATGCAGGCTGCATTGGGCGGCCTGCTGGGCCTGTTGGTTGGCAGCTTTCTCAACGTGGTGGT
ACATAGGCTGCCCCGCATGATGGAGCAGCAATGGAATGCGGAATGCGCCCAATGGGCGCAAGAGCAATCTTCATCCTCCG
CCACGCCATCGCCCGCCACAGCGCCGCCGCCTGCCGAGGCGCCCCTGAGCCTGAGCCGCCCCCGCTCGCGCTGCCCGCAT
TGCGGTCATGCCATCCACTGGTACGAGAATATTCCCGTGCTCAGTTATCTGGCGCTGCGAGGCCGGTGCTCCGAATGCAA
GGCGTCGATCAGCATCCGCTATCCGCTGGTGGAATTGGCCTGCGCCGCGCTGTTTGCCTACTGTGCGGCGCAATGGGGCA
TCACGCCTACAGGCGCTGCCTGGTGCCTTTTCAGTGCGGCCTTGCTGAGCCTGGCATTGATCGACTGGGACACCACACTG
CTTCCCGACGACATCACCCTGCCCTTGCTGTGGGCCGGACTGCTGTCGTCGGCGCTGCACTGGACCGCCGTGCCTCTGGC
CGACGCGGTATGGGGCGCTGCAGCGGGCTATCTCTCGCTGTGGCTGGTTTACTGGGCCTTCAAGCTGGCCACCGGCAAGG
AAGGCATGGGCTACGGCGACTTCAAGCTGTTTGCCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCACTCGTGCCCATCATC
CTGATCTCGTCGGTGATTGGCGCCATCGTGGGCATCGCGTTGAAGATCAACAGCCGCCTGCGTGAGGGCGGCTATGTTCC
CTTCGGCCCCTTTCTGGCGGGCGCCGGCTTTGCAGCCATGGTGTTCGGACCGCAGCAGATGCTGCAATCCCTATTGGCGG
CCTTGGGCCTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-131)

• Type IV leader peptidase family (Peptidase_A24) (143-252)

3D structure

• AlphaFold DB: A0A1H3L211

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.59	95.286	0.492
pilD	Vibrio campbellii strain DS40M4	46.416	98.653	0.458
pilD	Acinetobacter nosocomialis M2	44.364	92.593	0.411
pilD	Neisseria gonorrhoeae MS11	45.865	89.562	0.411
pilD	Acinetobacter baumannii D1279779	44	92.593	0.407