Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JQC68_RS08445 Genome accession   NZ_CP069308
Coordinates   1696043..1696624 (+) Length   193 a.a.
NCBI ID   WP_021110392.1    Uniprot ID   A0A084ELH5
Organism   Glaesserella parasuis strain EHP1804     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1691043..1701624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQC68_RS08405 (JQC68_08400) gltX 1691654..1693093 (-) 1440 WP_203398994.1 glutamate--tRNA ligase -
  JQC68_RS08430 (JQC68_08425) - 1693932..1694270 (+) 339 WP_021115585.1 selenocysteine synthase -
  JQC68_RS08435 (JQC68_08430) - 1694274..1694582 (+) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  JQC68_RS08440 (JQC68_08435) - 1694783..1695943 (+) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  JQC68_RS08445 (JQC68_08440) pilF 1696043..1696624 (+) 582 WP_021110392.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  JQC68_RS08450 (JQC68_08445) - 1696685..1697620 (+) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  JQC68_RS08455 (JQC68_08450) greB 1697620..1698099 (+) 480 WP_071610788.1 transcription elongation factor GreB -
  JQC68_RS08475 (JQC68_08470) - 1698635..1699933 (+) 1299 WP_021118667.1 NAD(P)/FAD-dependent oxidoreductase -
  JQC68_RS08480 (JQC68_08475) - 1700020..1700535 (-) 516 WP_203398995.1 TIGR00645 family protein -
  JQC68_RS08485 (JQC68_08480) - 1700738..1700998 (+) 261 WP_005714586.1 hypothetical protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.24 Da        Isoelectric Point: 8.0872

>NTDB_id=533476 JQC68_RS08445 WP_021110392.1 1696043..1696624(+) (pilF) [Glaesserella parasuis strain EHP1804]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=533476 JQC68_RS08445 WP_021110392.1 1696043..1696624(+) (pilF) [Glaesserella parasuis strain EHP1804]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A084ELH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

99.482

100

0.995
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415