Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   K9U07_RS18325 Genome accession   NZ_CP083512
Coordinates   3776065..3777447 (+) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain elppa4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3771065..3782447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U07_RS18300 (K9U07_18330) deoD 3772079..3772798 (+) 720 WP_000224879.1 purine-nucleoside phosphorylase -
  K9U07_RS18305 (K9U07_18335) - 3772959..3773222 (-) 264 WP_000566145.1 helix-turn-helix domain-containing protein -
  K9U07_RS18310 (K9U07_18340) lplA 3773254..3774270 (-) 1017 WP_000105849.1 lipoate--protein ligase LplA -
  K9U07_RS18315 (K9U07_18345) ytjB 3774298..3774942 (-) 645 WP_000124615.1 YtjB family periplasmic protein -
  K9U07_RS18320 (K9U07_18350) serB 3775048..3776016 (+) 969 WP_001132956.1 phosphoserine phosphatase -
  K9U07_RS18325 (K9U07_18355) radA/sms 3776065..3777447 (+) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  K9U07_RS18330 (K9U07_18360) nadR 3777468..3778700 (+) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  K9U07_RS18335 (K9U07_18365) - 3778972..3779088 (-) 117 Protein_3589 hypothetical protein -
  K9U07_RS18340 (K9U07_18370) - 3779161..3779373 (-) 213 WP_000848790.1 DUF1471 domain-containing protein -
  K9U07_RS18345 (K9U07_18375) - 3779531..3779947 (-) 417 WP_001140838.1 SRPBCC family protein -
  K9U07_RS18350 (K9U07_18380) - 3779973..3780770 (-) 798 WP_000762718.1 SDR family NAD(P)-dependent oxidoreductase -
  K9U07_RS18355 (K9U07_18385) - 3780790..3781800 (-) 1011 WP_000023619.1 aldo/keto reductase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=532690 K9U07_RS18325 WP_001029698.1 3776065..3777447(+) (radA/sms) [Escherichia coli strain elppa4]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=532690 K9U07_RS18325 WP_001029698.1 3776065..3777447(+) (radA/sms) [Escherichia coli strain elppa4]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAACTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTCGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATTGACTGTTCG
GTGCTTCTGGACGGCGATGCCGACTCCCGTTTCCGCACCTTGCGTAGCCATAAAAACCGCTTCGGCGCGGTGAATGAACT
GGGCGTCTTCGCGATGACCGAACAGGGTCTGCGTGAAGTCAGCAACCCTTCGGCAATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTAGTGTGGGAAGGAACGCGTCCGCTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTGGCGGTAGGGCTGGAGCAAAACCGTCTGGCGATCCTGCTGGCGGTGCTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCG
ACTTGGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGGCGGGC
GATTGTTCCGGCGGCTAATGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43