Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JNE45_RS11450 Genome accession   NZ_CP069061
Coordinates   2255836..2256906 (-) Length   356 a.a.
NCBI ID   WP_238409347.1    Uniprot ID   -
Organism   Bacillus safensis strain F6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2250836..2261906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNE45_RS11405 (JNE45_11405) sipW 2250931..2251503 (-) 573 WP_203128609.1 signal peptidase I SipW -
  JNE45_RS11410 (JNE45_11410) tapA 2251556..2252083 (-) 528 WP_203128610.1 amyloid fiber anchoring/assembly protein TapA -
  JNE45_RS11415 (JNE45_11415) - 2252383..2252661 (+) 279 WP_223251017.1 DUF3889 domain-containing protein -
  JNE45_RS11420 (JNE45_11420) - 2252695..2252889 (-) 195 WP_203128611.1 YqzE family protein -
  JNE45_RS11425 (JNE45_11425) - 2252949..2253332 (-) 384 WP_203128612.1 competence type IV pilus minor pilin ComGG -
  JNE45_RS11430 (JNE45_11430) comGF 2253329..2253856 (-) 528 WP_186318660.1 competence type IV pilus minor pilin ComGF -
  JNE45_RS19405 comGE 2253768..2254082 (-) 315 WP_111292285.1 competence type IV pilus minor pilin ComGE -
  JNE45_RS11435 (JNE45_11435) comGD 2254066..2254512 (-) 447 WP_197198255.1 competence type IV pilus minor pilin ComGD -
  JNE45_RS11440 (JNE45_11440) comGC 2254505..2254798 (-) 294 WP_048239211.1 competence type IV pilus major pilin ComGC Machinery gene
  JNE45_RS11445 (JNE45_11445) comGB 2254815..2255855 (-) 1041 WP_203128613.1 competence type IV pilus assembly protein ComGB -
  JNE45_RS11450 (JNE45_11450) comGA 2255836..2256906 (-) 1071 WP_238409347.1 competence type IV pilus ATPase ComGA Machinery gene
  JNE45_RS11455 (JNE45_11455) - 2257084..2257959 (-) 876 WP_095409007.1 STAS domain-containing protein -
  JNE45_RS11465 (JNE45_11465) - 2258183..2258563 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JNE45_RS11470 (JNE45_11470) - 2258776..2259018 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  JNE45_RS11475 (JNE45_11475) - 2259066..2259704 (-) 639 WP_095409008.1 MBL fold metallo-hydrolase -
  JNE45_RS11480 (JNE45_11480) - 2259862..2260035 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  JNE45_RS11485 (JNE45_11485) - 2260061..2260375 (-) 315 WP_048238883.1 MTH1187 family thiamine-binding protein -
  JNE45_RS11490 (JNE45_11490) - 2260452..2261705 (-) 1254 WP_095409009.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40424.93 Da        Isoelectric Point: 9.5040

>NTDB_id=532054 JNE45_RS11450 WP_238409347.1 2255836..2256906(-) (comGA) [Bacillus safensis strain F6]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELICPFCGETCQLYCKLNRPFRRTNVFELLYGKDLGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=532054 JNE45_RS11450 WP_238409347.1 2255836..2256906(-) (comGA) [Bacillus safensis strain F6]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCTTTCCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTGA
AGCATTCGCATGGACTCATTTTATTTACCGGTCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTATTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTGG
GAGAAATAAGAGATGCGGAAACGGCTAGAACGGCGATTAGGGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTTCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTCGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATTGAGCTTATATGCCCTTTTTGCGGGGAAACATGTCAGCTTTACTGTAAATTAAATCGTC
CTTTCAGACGAACAAATGTATTTGAATTGCTTTATGGAAAGGATCTTGGTGAGTGTATCAAAGAGGCCAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTACAAAGATTAATTCGGAAAGGAGTGGCACTCGGTTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649