Detailed information NTG0532033

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: JNE45_RS05245
• Genome accession: NZ_CP069061
• Coordinates: 1056938..1060642 (+)
• Length: 1234 a.a.
• NCBI ID: WP_203129532.1
• Uniprot ID: -
• Organism: Bacillus safensis strain F6
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1051938..1065642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JNE45_RS05230 (JNE45_05230)	-	1052243..1052653 (+)	411	WP_025093362.1	DoxX family protein	-
JNE45_RS05235 (JNE45_05235)	-	1052851..1053297 (-)	447	WP_048238419.1	ferritin-like domain-containing protein	-
JNE45_RS05240 (JNE45_05240)	addB	1053451..1056960 (+)	3510	WP_203129527.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
JNE45_RS05245 (JNE45_05245)	addA	1056938..1060642 (+)	3705	WP_203129532.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
JNE45_RS05250 (JNE45_05250)	sbcD	1060669..1061844 (+)	1176	WP_144470097.1	exonuclease subunit SbcD	-
JNE45_RS05255 (JNE45_05255)	-	1061841..1065236 (+)	3396	WP_203129534.1	AAA family ATPase	-
JNE45_RS05260 (JNE45_05260)	-	1065256..1065555 (+)	300	WP_203129536.1	HNH endonuclease	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142403.65 Da
• Isoelectric Point: 5.1148

>NTDB_id=532033 JNE45_RS05245 WP_203129532.1 1056938..1060642(+) (addA) [Bacillus safensis strain F6]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEEELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGQHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFKPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLEQFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLIPDFERYQSKTYLDFIGPALIRHQAMSSILEETGNVVLSHPSLFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRRAR
TTLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLPSDVLYDDSRIQQLLDSLQQRDLLTNEQVHSIDQEGITAFFSTSIGQ
KLKKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAAPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=532033 JNE45_RS05245 WP_203129532.1 1056938..1060642(+) (addA) [Bacillus safensis strain F6]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAACATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCACTCATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGCTTACAGGTGCTGCGTACCTTTTACTATGAGATTGACCTTGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGAAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTATACTTCTGACCGTCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTATCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCT
CTCGCTCTATCAAAAGAACCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
GATCAGGCATCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATTAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTCAAA
GATGAATACTTTATGCGAAGTCCCGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCAGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGATTTGAAAGAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCGGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAAGGGCA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTAAACCAGATGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGTAAAGATACAACGACAGAAATGCTCCTTGTTCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGGGAAGAACTAGAGACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTAGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAATTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGGATTCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCTATTGTTCATTTAGACGAAAATGAAATGGCACTGATCCGCACGAGTGA
TAAGAAAGGCACTTATTATGATGCTGTGAAAGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACAATTTTTCAGGTTGCTGCGTAAGTGGCGTGATTTCTCTATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGCGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTACTTGCTTGA
TAAAGAATTAGGATTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAACTATTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGACTGGCTGAT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTTATCCGTCACCAAGCGATGTCAT
CTATTTTAGAAGAAACAGGTAATGTCGTTCTGTCGCATCCATCATTATTCACGATCTCATTTACTCAAGCTTCTGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATATCCATATTTAGCGGCCTCACAAGTCGGTACAA
AGCAGTCTGTTTCTGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAGAGCTCGT
ACAACGTTGTATGACCGTCCGGCTTTCATGAAGAAAAAAGCACTGACTGCTGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAGCATATTCCGCTTCCTTCTGATGTGCTCTATGATGATTCACGTATACAGCAACTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAACAAGTCCATTCAATTGATCAAGAAGGAATTACAGCTTTCTTCTCAACTTCTATTGGTCAA
AAGCTGAAAAAAGCGGATTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGAGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGCAGATATACAGGCGGCCTTGAAGCAGCTGCGCCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
CGGGAATTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.181	100	0.663