Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   JNE45_RS05240 Genome accession   NZ_CP069061
Coordinates   1053451..1056960 (+) Length   1169 a.a.
NCBI ID   WP_203129527.1    Uniprot ID   -
Organism   Bacillus safensis strain F6     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1048451..1061960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNE45_RS05210 (JNE45_05210) lepB 1048969..1049481 (+) 513 WP_047202354.1 signal peptidase I -
  JNE45_RS05215 (JNE45_05215) - 1049519..1050664 (-) 1146 WP_203129959.1 AbrB family transcriptional regulator -
  JNE45_RS05220 (JNE45_05220) - 1050931..1051353 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  JNE45_RS05225 (JNE45_05225) - 1051346..1052185 (+) 840 WP_203129525.1 M56 family metallopeptidase -
  JNE45_RS05230 (JNE45_05230) - 1052243..1052653 (+) 411 WP_025093362.1 DoxX family protein -
  JNE45_RS05235 (JNE45_05235) - 1052851..1053297 (-) 447 WP_048238419.1 ferritin-like domain-containing protein -
  JNE45_RS05240 (JNE45_05240) addB 1053451..1056960 (+) 3510 WP_203129527.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  JNE45_RS05245 (JNE45_05245) addA 1056938..1060642 (+) 3705 WP_203129532.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  JNE45_RS05250 (JNE45_05250) sbcD 1060669..1061844 (+) 1176 WP_144470097.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134765.75 Da        Isoelectric Point: 5.3923

>NTDB_id=532032 JNE45_RS05240 WP_203129527.1 1053451..1056960(+) (addB) [Bacillus safensis strain F6]
MEIQFLAGRSGSGKTTAILEEIKEQLRHDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITATFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLARECGAAISENILEGNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGAAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHQHALYVLDPEQLGDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=532032 JNE45_RS05240 WP_203129527.1 1053451..1056960(+) (addB) [Bacillus safensis strain F6]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCATGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGCGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACGGAAGAAAGACGGGCAGCTGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCCGAAGACTATTT
GACGCTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCCCACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACTGCTACGTTCACAGTGGATCGTTCC
TATCATGATAAGCAACCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCAAG
AGAGTGCGGAGCGGCCATTTCTGAGAACATTTTGGAGGGAAATAACCGTCATCTTCATACGCCAGATCTTGCTTATTTAG
AACGCCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTAACCCCTCATCTCACAGTATCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAGGGAGCAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGGCTCAGACTGCGGGATATTTCGAT
TGTAGCGAGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTATGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGCTGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGTTTCCATTACAGACGAT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCCCCGCTTTTCCAGTTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTCAAAGACATGGTGGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCGGAAAAACTTGAAAAGGCCAGGCTGGAGGCTGAGGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTCATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGACTCGCCTCTCTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAGTGCATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTTTTGTCTGAGGATGACCGGGAATGGTTAAAGCGAGCTGGAGCAGAGCTCGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGCTTTCGAGTCCATCCCATCATTTGTATCT
GTCTTATTCGGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCGA
ATCATCAGCATGCTTTGTATGTGTTAGACCCAGAACAGCTGGGTGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCTTATACCGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATCAGTGGTGTGTGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTTTCGAAAAATATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
GCTTAAAACCGAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCGTTTTCTCACTTTGCTTCCCACGGGTTAAAATTAAAGGACCGGCAATTTTATAAATTGGAAGCGCCTGATATCGG
CCAGCTGTTTCACTCAGCTTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGACTGTGTCACTTATTCAAGACATGCAATCGAACAACTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATCTTAAGTGAGCATGCAAAAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGTCAAGGACCTTTGCCACCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTTGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATATTATGGACTGGCCTTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAGGAATGGCTCGGCGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAAGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTTCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGTGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTCGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.236

99.316

0.658