Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DI291_RS13345 Genome accession   NZ_CP068988
Coordinates   2672816..2673880 (-) Length   354 a.a.
NCBI ID   WP_095291091.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain SUBG0010     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2667816..2678880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DI291_RS13300 (DI291_13695) tapA 2668311..2669039 (-) 729 WP_205802129.1 amyloid fiber anchoring/assembly protein TapA -
  DI291_RS13305 (DI291_13700) - 2669316..2669636 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  DI291_RS13310 (DI291_13705) - 2669666..2669848 (-) 183 WP_020452171.1 YqzE family protein -
  DI291_RS13315 (DI291_13710) comGG 2669937..2670302 (-) 366 WP_025811163.1 competence type IV pilus minor pilin ComGG -
  DI291_RS13320 (DI291_13715) comGF 2670314..2670802 (-) 489 WP_229128989.1 competence type IV pilus minor pilin ComGF -
  DI291_RS13325 (DI291_13720) comGE 2670711..2671058 (-) 348 WP_025811161.1 competence type IV pilus minor pilin ComGE -
  DI291_RS13330 (DI291_13725) comGD 2671042..2671479 (-) 438 WP_095291094.1 competence type IV pilus minor pilin ComGD -
  DI291_RS13335 (DI291_13730) comGC 2671485..2671778 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  DI291_RS13340 (DI291_13735) comGB 2671792..2672826 (-) 1035 WP_035338372.1 competence type IV pilus assembly protein ComGB Machinery gene
  DI291_RS13345 (DI291_13740) comGA 2672816..2673880 (-) 1065 WP_095291091.1 competence type IV pilus ATPase ComGA Machinery gene
  DI291_RS13350 (DI291_13745) - 2674038..2674877 (-) 840 WP_020452179.1 STAS domain-containing protein -
  DI291_RS13360 (DI291_13755) - 2675119..2675496 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DI291_RS13365 (DI291_13760) - 2675707..2675952 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  DI291_RS13370 (DI291_13765) - 2675988..2676626 (-) 639 WP_095291088.1 MBL fold metallo-hydrolase -
  DI291_RS13375 (DI291_13770) - 2676782..2676955 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  DI291_RS13380 (DI291_13775) - 2677016..2677330 (-) 315 WP_095291085.1 MTH1187 family thiamine-binding protein -
  DI291_RS13385 (DI291_13780) - 2677346..2678455 (-) 1110 WP_105981078.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39824.31 Da        Isoelectric Point: 9.1063

>NTDB_id=531852 DI291_RS13345 WP_095291091.1 2672816..2673880(-) (comGA) [Bacillus paralicheniformis strain SUBG0010]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLILFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDNCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GDIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=531852 DI291_RS13345 WP_095291091.1 2672816..2673880(-) (comGA) [Bacillus paralicheniformis strain SUBG0010]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTTCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGATATTGGGGAAAGACGGCTGCCGCAAAGCGGAGCTTTGACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATTCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATCGAATAC
GCTAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTTGAATCAAGAAGCGAAAACATTTTGCAAGTGCA
AGTAAACGAAAAAGCTGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTCG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCCAAAGGCGCAATATACAGGCTGCTCGAGTTCGGCGTTGATATGACAGAAATTGAACAAACACTGGTCGC
AATCAGCGCCCAGCGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAACTGCTCTTTATATTGCAGATTGTCGAGGC
CTGTGAGAAGAGCAAGCGTCTTCGAGCTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC
GGTGACATAAAAACAGAAACATTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.797

100

0.678
  pilB Vibrio cholerae strain A1552

38.671

93.503

0.362