Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JM957_RS21230 Genome accession   NZ_CP068724
Coordinates   4140768..4141811 (-) Length   347 a.a.
NCBI ID   WP_001013170.1    Uniprot ID   A0A9Q5QS48
Organism   Bacillus cereus strain AFA01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4135768..4146811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JM957_RS30275 - 4135833..4135937 (+) 105 Protein_4160 IS3 family transposase -
  JM957_RS21185 - 4136201..4136401 (-) 201 WP_000106083.1 YqzE family protein -
  JM957_RS21190 - 4136440..4136937 (-) 498 WP_059303452.1 shikimate kinase -
  JM957_RS21195 - 4137053..4137703 (-) 651 WP_065382056.1 2OG-Fe(II) oxygenase -
  JM957_RS21200 comGG 4137878..4138249 (-) 372 WP_001231316.1 competence type IV pilus minor pilin ComGG -
  JM957_RS21205 comGF 4138246..4138716 (-) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  JM957_RS21210 - 4138686..4138988 (-) 303 WP_000829469.1 hypothetical protein -
  JM957_RS21215 comGD 4138981..4139436 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  JM957_RS21220 comGC 4139433..4139732 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  JM957_RS21225 comGB 4139744..4140781 (-) 1038 WP_065382055.1 competence type IV pilus assembly protein ComGB -
  JM957_RS21230 comGA 4140768..4141811 (-) 1044 WP_001013170.1 competence type IV pilus ATPase ComGA Machinery gene
  JM957_RS21235 - 4142017..4142712 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  JM957_RS21240 - 4142838..4143080 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  JM957_RS21245 - 4143188..4144582 (+) 1395 WP_001094345.1 L-cystine transporter -
  JM957_RS21250 - 4144661..4145053 (-) 393 WP_065382054.1 hypothetical protein -
  JM957_RS21255 - 4145141..4145356 (-) 216 WP_001008327.1 DUF3912 family protein -
  JM957_RS21260 - 4145609..4145920 (+) 312 WP_001093237.1 hypothetical protein -
  JM957_RS21265 - 4145955..4146443 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=530756 JM957_RS21230 WP_001013170.1 4140768..4141811(-) (comGA) [Bacillus cereus strain AFA01]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=530756 JM957_RS21230 WP_001013170.1 4140768..4141811(-) (comGA) [Bacillus cereus strain AFA01]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTCCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372