Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JHT10_RS21365 Genome accession   NZ_CP068631
Coordinates   2773026..2773619 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain TJ-20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2768026..2778619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHT10_RS21345 (JHT10_21305) aroB 2768902..2770002 (-) 1101 WP_005496576.1 3-dehydroquinate synthase -
  JHT10_RS21350 (JHT10_21310) aroK 2770034..2770552 (-) 519 WP_005381319.1 shikimate kinase AroK -
  JHT10_RS21355 (JHT10_21315) pilQ 2770741..2772489 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  JHT10_RS21360 (JHT10_21320) pilP 2772521..2773036 (-) 516 WP_017449464.1 pilus assembly protein PilP Machinery gene
  JHT10_RS21365 (JHT10_21325) pilO 2773026..2773619 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHT10_RS21370 (JHT10_21330) pilN 2773612..2774190 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  JHT10_RS21375 (JHT10_21335) pilM 2774174..2775190 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  JHT10_RS21380 (JHT10_21340) - 2775333..2777903 (+) 2571 WP_029804285.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=530091 JHT10_RS21365 WP_005480482.1 2773026..2773619(-) (pilO) [Vibrio parahaemolyticus strain TJ-20]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=530091 JHT10_RS21365 WP_005480482.1 2773026..2773619(-) (pilO) [Vibrio parahaemolyticus strain TJ-20]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726