Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B73_RS01500 Genome accession   NZ_CP082827
Coordinates   330537..332033 (+) Length   498 a.a.
NCBI ID   WP_001040619.1    Uniprot ID   -
Organism   Escherichia coli strain SCAID WND2-2021 (3/145)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325537..337033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B73_RS01470 (K8B73_01470) - 325790..326098 (+) 309 WP_000385056.1 hypothetical protein -
  K8B73_RS01475 (K8B73_01475) zntA 326131..328329 (-) 2199 WP_000106497.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  K8B73_RS01480 (K8B73_01480) yhhN 328403..329029 (-) 627 WP_000964718.1 lysoplasmalogenase -
  K8B73_RS01485 (K8B73_01485) yhhM 329170..329529 (+) 360 WP_000042883.1 DUF2500 domain-containing protein -
  K8B73_RS01490 (K8B73_01490) yhhL 329532..329801 (-) 270 WP_001295207.1 DUF1145 family protein -
  K8B73_RS01495 (K8B73_01495) rsmD 329791..330387 (-) 597 WP_000743195.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  K8B73_RS01500 (K8B73_01500) pilA 330537..332033 (+) 1497 WP_001040619.1 signal recognition particle-docking protein FtsY Machinery gene
  K8B73_RS01505 (K8B73_01505) ftsE 332036..332704 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  K8B73_RS01510 (K8B73_01510) ftsX 332697..333755 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  K8B73_RS01515 (K8B73_01515) rpoH 334000..334854 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  K8B73_RS01520 (K8B73_01520) livJ 335126..336229 (+) 1104 WP_001021986.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  K8B73_RS01525 (K8B73_01525) panM 336429..336812 (-) 384 WP_000778800.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54668.50 Da        Isoelectric Point: 4.1468

>NTDB_id=530076 K8B73_RS01500 WP_001040619.1 330537..332033(+) (pilA) [Escherichia coli strain SCAID WND2-2021 (3/145)]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVVIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=530076 K8B73_RS01500 WP_001040619.1 330537..332033(+) (pilA) [Escherichia coli strain SCAID WND2-2021 (3/145)]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGTTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAACCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAGCAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAGGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTTCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378