Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JHS95_RS01590 Genome accession   NZ_CP068627
Coordinates   335315..336331 (+) Length   338 a.a.
NCBI ID   WP_237301910.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain LH80     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330315..341331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHS95_RS01575 (JHS95_01580) - 330739..331467 (-) 729 WP_025442305.1 redoxin family protein -
  JHS95_RS01580 (JHS95_01585) oxyR 331638..332546 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  JHS95_RS01585 (JHS95_01590) - 332602..335172 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  JHS95_RS01590 (JHS95_01595) pilM 335315..336331 (+) 1017 WP_237301910.1 type IV pilus biogenesis protein PilM Machinery gene
  JHS95_RS01595 (JHS95_01600) pilN 336315..336893 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  JHS95_RS01600 (JHS95_01605) pilO 336886..337479 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHS95_RS01605 (JHS95_01610) pilP 337469..337984 (+) 516 WP_015297395.1 pilus assembly protein PilP Machinery gene
  JHS95_RS01610 (JHS95_01615) pilQ 338016..339761 (+) 1746 WP_237301911.1 type IV pilus secretin PilQ Machinery gene
  JHS95_RS01615 (JHS95_01620) aroK 339950..340468 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37794.34 Da        Isoelectric Point: 6.8323

>NTDB_id=530014 JHS95_RS01590 WP_237301910.1 335315..336331(+) (pilM) [Vibrio parahaemolyticus strain LH80]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAMVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=530014 JHS95_RS01590 WP_237301910.1 335315..336331(+) (pilM) [Vibrio parahaemolyticus strain LH80]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGATGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCGTTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.071

99.704

0.479