Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   JKJ03_RS12035 Genome accession   NZ_CP068563
Coordinates   2492068..2492835 (-) Length   255 a.a.
NCBI ID   WP_071391600.1    Uniprot ID   -
Organism   Bacillus velezensis strain GUMT319     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2487068..2497835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKJ03_RS12015 (JKJ03_12015) - 2487270..2488082 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  JKJ03_RS12020 (JKJ03_12020) ccpN 2488108..2488746 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  JKJ03_RS12025 (JKJ03_12025) glyS 2488847..2490886 (-) 2040 WP_202735633.1 glycine--tRNA ligase subunit beta -
  JKJ03_RS12030 (JKJ03_12030) glyQ 2490879..2491766 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  JKJ03_RS12035 (JKJ03_12035) recO 2492068..2492835 (-) 768 WP_071391600.1 DNA repair protein RecO Machinery gene
  JKJ03_RS12040 (JKJ03_12040) - 2492872..2493015 (-) 144 WP_007408286.1 YqzL family protein -
  JKJ03_RS12045 (JKJ03_12045) era 2493171..2494076 (-) 906 WP_007408285.1 GTPase Era -
  JKJ03_RS12050 (JKJ03_12050) - 2494057..2494467 (-) 411 WP_003152976.1 cytidine deaminase -
  JKJ03_RS12055 (JKJ03_12055) - 2494591..2494965 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  JKJ03_RS12060 (JKJ03_12060) ybeY 2494946..2495419 (-) 474 WP_202735634.1 rRNA maturation RNase YbeY -
  JKJ03_RS12065 (JKJ03_12065) - 2495420..2497552 (-) 2133 WP_015387990.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29273.87 Da        Isoelectric Point: 8.3139

>NTDB_id=529475 JKJ03_RS12035 WP_071391600.1 2492068..2492835(-) (recO) [Bacillus velezensis strain GUMT319]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKHVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=529475 JKJ03_RS12035 WP_071391600.1 2492068..2492835(-) (recO) [Bacillus velezensis strain GUMT319]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CGAAAAACGAAATTAAACATGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.02

100

0.89