Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   K8B89_RS15670 Genome accession   NZ_CP082775
Coordinates   3081290..3082714 (+) Length   474 a.a.
NCBI ID   WP_001576845.1    Uniprot ID   -
Organism   Escherichia coli O25:H4 strain CU568-3     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3076290..3087714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B89_RS15620 (K8B89_15500) - 3076314..3076697 (+) 384 WP_000951323.1 hypothetical protein -
  K8B89_RS15625 (K8B89_15505) - 3076721..3077014 (+) 294 WP_001111302.1 phage anti-RecBCD protein -
  K8B89_RS15630 (K8B89_15510) - 3077025..3077189 (+) 165 WP_001576836.1 DUF2737 family protein -
  K8B89_RS15635 (K8B89_15515) - 3077186..3077659 (+) 474 WP_001576837.1 hypothetical protein -
  K8B89_RS15640 (K8B89_15520) - 3077656..3078324 (+) 669 WP_001576838.1 ead/Ea22-like family protein -
  K8B89_RS15645 (K8B89_15525) - 3078677..3078901 (+) 225 Protein_3052 DUF551 domain-containing protein -
  K8B89_RS15650 (K8B89_15530) - 3078894..3079178 (+) 285 WP_001576840.1 ASCH domain-containing protein -
  K8B89_RS15655 (K8B89_15535) - 3079251..3079418 (+) 168 WP_001576841.1 hypothetical protein -
  K8B89_RS15660 (K8B89_15540) - 3079758..3079949 (+) 192 WP_001576843.1 hypothetical protein -
  K8B89_RS15665 (K8B89_15545) - 3079930..3081108 (-) 1179 WP_024188919.1 site-specific integrase -
  K8B89_RS15670 (K8B89_15550) sbcB 3081290..3082714 (+) 1425 WP_001576845.1 exodeoxyribonuclease I Machinery gene
  K8B89_RS15675 (K8B89_15555) tnpA 3082825..3083283 (-) 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -
  K8B89_RS15680 (K8B89_15560) plaP 3083500..3084858 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  K8B89_RS15685 (K8B89_15565) yoeI 3084848..3084910 (-) 63 WP_010723108.1 membrane protein YoeI -
  K8B89_RS15690 (K8B89_15570) yeeY 3085125..3086054 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  K8B89_RS15695 (K8B89_15575) yeeZ 3086100..3086924 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  K8B89_RS15700 (K8B89_15580) yoeB 3087007..3087261 (-) 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  K8B89_RS15705 (K8B89_15585) yefM 3087258..3087509 (-) 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54334.56 Da        Isoelectric Point: 5.3476

>NTDB_id=528872 K8B89_RS15670 WP_001576845.1 3081290..3082714(+) (sbcB) [Escherichia coli O25:H4 strain CU568-3]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVILVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=528872 K8B89_RS15670 WP_001576845.1 3081290..3082714(+) (sbcB) [Escherichia coli O25:H4 strain CU568-3]
ATGACAGACACAGATAAGCAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAACGAA
GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTAGTTCACGTTTCCGGAATGTTTGGGGCATGGCGTGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTTTGGTGGATTTGGCAGGAGACAT
TTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACACCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACACTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTATGCTGATGAATTGCAGATGCTGGCACAACAGTATG
CCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

99.156

0.586