Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   K8B88_RS20735 Genome accession   NZ_CP082774
Coordinates   4244699..4245358 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O16:H6 strain CU37RT-2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4239699..4250358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B88_RS20720 (K8B88_20645) ygiS 4241904..4243511 (-) 1608 WP_001468194.1 ABC transporter substrate-binding protein -
  K8B88_RS20725 (K8B88_20650) ygiV 4243620..4244102 (-) 483 WP_021538160.1 GyrI-like domain-containing protein -
  K8B88_RS20730 (K8B88_20655) ygiW 4244155..4244547 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  K8B88_RS20735 (K8B88_20660) ciaR 4244699..4245358 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  K8B88_RS20740 (K8B88_20665) qseC 4245355..4246704 (+) 1350 WP_000673388.1 quorum sensing histidine kinase QseC -
  K8B88_RS20745 (K8B88_20670) ygiZ 4246750..4247082 (-) 333 WP_000914696.1 DUF2645 family protein -
  K8B88_RS20750 - 4247089..4247799 (-) 711 WP_000834024.1 hypothetical protein -
  K8B88_RS20755 - 4247802..4248281 (-) 480 WP_000069056.1 Hcp family type VI secretion system effector -
  K8B88_RS20760 (K8B88_20680) mdaB 4248531..4249112 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  K8B88_RS20765 (K8B88_20685) ygiN 4249143..4249457 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=528808 K8B88_RS20735 WP_001221493.1 4244699..4245358(+) (ciaR) [Escherichia coli O16:H6 strain CU37RT-2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=528808 K8B88_RS20735 WP_001221493.1 4244699..4245358(+) (ciaR) [Escherichia coli O16:H6 strain CU37RT-2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365