Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JKL50_RS00825 Genome accession   NZ_CP068389
Coordinates   142144..143214 (+) Length   356 a.a.
NCBI ID   WP_144668081.1    Uniprot ID   -
Organism   Bacillus altitudinis strain ws31     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137144..148214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKL50_RS00790 (JKL50_00790) - 138396..138710 (+) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -
  JKL50_RS00795 (JKL50_00795) - 138735..138908 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  JKL50_RS00800 (JKL50_00800) - 139069..139707 (+) 639 WP_202734344.1 MBL fold metallo-hydrolase -
  JKL50_RS00805 (JKL50_00805) - 139756..139998 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  JKL50_RS00810 (JKL50_00810) - 140211..140591 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JKL50_RS00820 (JKL50_00820) - 140817..141686 (+) 870 WP_007501231.1 STAS domain-containing protein -
  JKL50_RS00825 (JKL50_00825) comGA 142144..143214 (+) 1071 WP_144668081.1 competence type IV pilus ATPase ComGA Machinery gene
  JKL50_RS00830 (JKL50_00830) comGB 143195..144235 (+) 1041 WP_035390815.1 competence type IV pilus assembly protein ComGB -
  JKL50_RS00835 (JKL50_00835) comGC 144252..144545 (+) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  JKL50_RS00840 (JKL50_00840) comGD 144538..144984 (+) 447 WP_202734345.1 competence type IV pilus minor pilin ComGD -
  JKL50_RS19410 - 145004..145282 (+) 279 WP_255234198.1 type II secretion system protein -
  JKL50_RS00845 (JKL50_00845) comGF 145224..145721 (+) 498 WP_046343974.1 competence type IV pilus minor pilin ComGF -
  JKL50_RS00850 (JKL50_00850) - 145718..146101 (+) 384 WP_046343972.1 competence type IV pilus minor pilin ComGG -
  JKL50_RS00855 (JKL50_00855) - 146162..146356 (+) 195 WP_008342191.1 YqzE family protein -
  JKL50_RS00860 (JKL50_00860) - 146391..146717 (-) 327 WP_039166025.1 DUF3889 domain-containing protein -
  JKL50_RS00865 (JKL50_00865) tapA 146970..147497 (+) 528 WP_046527018.1 amyloid fiber anchoring/assembly protein TapA -
  JKL50_RS00870 (JKL50_00870) sipW 147547..148119 (+) 573 WP_041091749.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40334.66 Da        Isoelectric Point: 9.0449

>NTDB_id=528808 JKL50_RS00825 WP_144668081.1 142144..143214(+) (comGA) [Bacillus altitudinis strain ws31]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTVNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=528808 JKL50_RS00825 WP_144668081.1 142144..143214(+) (comGA) [Bacillus altitudinis strain ws31]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGGCCACAAAATGGATCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGGTGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.607

100

0.646