Detailed information NTG0528737

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: JK151_RS15110
• Genome accession: NZ_CP068285
• Coordinates: 3323404..3324285 (+)
• Length: 293 a.a.
• NCBI ID: WP_078222781.1
• Uniprot ID: -
• Organism: Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3318404..3329285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JK151_RS15095 (JK151_15095)	-	3318839..3320116 (+)	1278	WP_013211407.1	HlyC/CorC family transporter	-
JK151_RS15100 (JK151_15100)	pilF	3320364..3322091 (+)	1728	WP_078222783.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JK151_RS15105 (JK151_15105)	-	3322136..3323401 (+)	1266	WP_078222782.1	type II secretion system F family protein	-
JK151_RS15110 (JK151_15110)	pilD	3323404..3324285 (+)	882	WP_078222781.1	prepilin peptidase	Machinery gene
JK151_RS15115 (JK151_15115)	coaE	3324298..3324912 (+)	615	WP_078222780.1	dephospho-CoA kinase	-
JK151_RS15120 (JK151_15120)	zapD	3325030..3325788 (+)	759	WP_013211402.1	cell division protein ZapD	-
JK151_RS15125 (JK151_15125)	-	3325800..3326015 (+)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
JK151_RS15130 (JK151_15130)	-	3326012..3326470 (-)	459	WP_048817320.1	NUDIX domain-containing protein	-
JK151_RS15135 (JK151_15135)	-	3326448..3327332 (-)	885	WP_013211399.1	ATP-binding protein	-
JK151_RS15140 (JK151_15140)	argJ	3327348..3328577 (-)	1230	WP_078222779.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31663.68 Da
• Isoelectric Point: 8.0336

>NTDB_id=528737 JK151_RS15110 WP_078222781.1 3323404..3324285(+) (pilD) [Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01]
MSGMFVIPTLAQLPAWFVIGSAALLGLLIGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH
QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=528737 JK151_RS15110 WP_078222781.1 3323404..3324285(+) (pilD) [Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01]
ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT
GCTGATCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT
TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG
TAGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCTGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

• Type IV leader peptidase family (Peptidase_A24) (140-247)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	46.617	90.785	0.423
pilD	Acinetobacter baumannii D1279779	46.617	90.785	0.423