Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   K8B90_RS21195 Genome accession   NZ_CP082771
Coordinates   4358393..4359631 (-) Length   412 a.a.
NCBI ID   WP_000816011.1    Uniprot ID   A0A0H2Z3C0
Organism   Escherichia coli LF82     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4353393..4364631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B90_RS21175 (K8B90_21075) dam 4354005..4354841 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  K8B90_RS21180 (K8B90_21080) damX 4354948..4356234 (-) 1287 WP_000343174.1 cell division protein DamX -
  K8B90_RS21185 (K8B90_21085) aroB 4356326..4357414 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  K8B90_RS21190 (K8B90_21090) aroK 4357471..4357992 (-) 522 WP_000818618.1 shikimate kinase AroK -
  K8B90_RS21195 (K8B90_21095) comE 4358393..4359631 (-) 1239 WP_000816011.1 DNA uptake porin HofQ Machinery gene
  K8B90_RS21200 (K8B90_21100) hofP 4359543..4359947 (-) 405 WP_001264138.1 DNA utilization protein HofP -
  K8B90_RS21205 (K8B90_21105) hofO 4359937..4360377 (-) 441 WP_001055746.1 DNA utilization protein HofO -
  K8B90_RS21210 (K8B90_21110) hofN 4360361..4360900 (-) 540 WP_001069307.1 DNA utilization protein HofN -
  K8B90_RS21215 (K8B90_21115) hofM 4360900..4361679 (-) 780 WP_001296474.1 DNA utilization protein HofM -
  K8B90_RS21220 (K8B90_21120) mrcA 4361799..4364351 (+) 2553 WP_001298208.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44839.34 Da        Isoelectric Point: 5.9488

>NTDB_id=528552 K8B90_RS21195 WP_000816011.1 4358393..4359631(-) (comE) [Escherichia coli LF82]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQVLAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=528552 K8B90_RS21195 WP_000816011.1 4358393..4359631(-) (comE) [Escherichia coli LF82]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGTGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTCTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364