Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   K7X33_RS21490 Genome accession   NZ_CP082355
Coordinates   4519710..4521908 (+) Length   732 a.a.
NCBI ID   WP_001309112.1    Uniprot ID   -
Organism   Escherichia coli strain VCSW5     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4514710..4526908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7X33_RS21470 (K7X33_21470) metB 4516812..4517972 (-) 1161 WP_001295694.1 cystathionine gamma-synthase -
  K7X33_RS21475 (K7X33_21475) metJ 4518249..4518566 (+) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  K7X33_RS21480 (K7X33_21480) yiiX 4518626..4519234 (+) 609 WP_000702310.1 YiiX family permuted papain-like enzyme -
  K7X33_RS21485 (K7X33_21485) rpmE 4519295..4519507 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  K7X33_RS21490 (K7X33_21490) priA 4519710..4521908 (+) 2199 WP_001309112.1 primosomal protein N' Machinery gene
  K7X33_RS21495 (K7X33_21495) cytR 4522064..4523089 (+) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  K7X33_RS21500 (K7X33_21500) ftsN 4523181..4524140 (+) 960 WP_000068834.1 cell division protein FtsN -
  K7X33_RS21505 (K7X33_21505) hslV 4524233..4524763 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  K7X33_RS21510 (K7X33_21510) hslU 4524773..4526104 (+) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81684.98 Da        Isoelectric Point: 9.0294

>NTDB_id=528389 K7X33_RS21490 WP_001309112.1 4519710..4521908(+) (priA) [Escherichia coli strain VCSW5]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=528389 K7X33_RS21490 WP_001309112.1 4519710..4521908(+) (priA) [Escherichia coli strain VCSW5]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGCG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCCAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGTGATCAGGTCGCGGAACTTGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAACGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGTGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATTTGAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGTTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGTTCCGCAACGCCCGCGCTGGAAACGTTGTGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCAGGGAATGCGCGGCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTACAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAACCG
CCGAGGGTTTGCGCCTGCGCTACTGTGCCACGACTGTGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGCCACCATTGCGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCTTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCACCGTTGTTCCCTGGTGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGTAAAGGTGCGCTGGAACAACAACTGGCGGAAGTACATCGCGGTGGCGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGACGTGACGCTGGTAGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTTGCCCAGCTTTATACCCAGGTGGCAGGCCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCATCATCCAGAACACCCGCTATTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGACGCATGATGCAGCTGCCGCCATGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCGCTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAAATTTTGTTGC
AGCACCCTTCCCGCGTGCGATTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(203-362)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.912

100

0.452