Detailed information NTG0052709

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PCC21_RS14425
• Genome accession: NC_018525
• Coordinates: 3287930..3288775 (-)
• Length: 281 a.a.
• NCBI ID: WP_014916092.1
• Uniprot ID: -
• Organism: Pectobacterium carotovorum subsp. carotovorum PCC21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3282930..3293775

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCC21_RS14405 (PCC21_029180)	-	3283150..3283920 (-)	771	WP_014916088.1	ABC transporter ATP-binding protein	-
PCC21_RS14410 (PCC21_029190)	-	3283922..3284941 (-)	1020	WP_014916089.1	FecCD family ABC transporter permease	-
PCC21_RS14415 (PCC21_029200)	-	3284938..3286002 (-)	1065	WP_014916090.1	ABC transporter substrate-binding protein	-
PCC21_RS14420 (PCC21_029210)	-	3286312..3287337 (+)	1026	WP_014916091.1	LLM class flavin-dependent oxidoreductase	-
PCC21_RS22210	ypdK	3287695..3287769 (+)	75	WP_099606229.1	membrane protein YpdK	-
PCC21_RS14425 (PCC21_029220)	pilD	3287930..3288775 (-)	846	WP_014916092.1	prepilin peptidase	Machinery gene
PCC21_RS14430 (PCC21_029230)	-	3288884..3289630 (-)	747	WP_010681640.1	type II secretion system protein N	-
PCC21_RS14435 (PCC21_029240)	gspM	3289620..3290114 (-)	495	WP_014916093.1	type II secretion system protein GspM	-
PCC21_RS14440 (PCC21_029250)	gspL	3290111..3291388 (-)	1278	WP_043901894.1	type II secretion system protein GspL	-
PCC21_RS14445 (PCC21_029260)	gspK	3291385..3292371 (-)	987	WP_014916095.1	type II secretion system minor pseudopilin GspK	-
PCC21_RS14450 (PCC21_029270)	gspJ	3292368..3292979 (-)	612	WP_014916096.1	type II secretion system minor pseudopilin GspJ	-
PCC21_RS14455 (PCC21_029280)	gspI	3293018..3293383 (-)	366	WP_039273738.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31401.49 Da
• Isoelectric Point: 7.6273

>NTDB_id=52709 PCC21_RS14425 WP_014916092.1 3287930..3288775(-) (pilD) [Pectobacterium carotovorum subsp. carotovorum PCC21]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSAPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCRQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSETFVPLIDAVVGAMAGYLSLWLLYRVFKYVTGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=52709 PCC21_RS14425 WP_014916092.1 3287930..3288775(-) (pilD) [Pectobacterium carotovorum subsp. carotovorum PCC21]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCCTACCGATTATGCTGGAGCGTCGCTGGCGGCAGGATATCGAACTTGAAACGG
GCATGGCCGATTCCGCTCCTGATACACGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGTTGCGTGGCCGCTCCCGCTGCTGTCGTCAATCGGTGTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGTTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGCTGCTGTTTAATCTGTCGGAGACCTTTGTCCCATTGATTGATGCCGTAGTCGGCGC
GATGGCTGGTTATTTATCTCTGTGGCTACTTTATCGGGTATTCAAATACGTAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGTTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTTGTTACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.815	96.441	0.48
pilD	Neisseria gonorrhoeae MS11	46.743	92.883	0.434
pilD	Vibrio campbellii strain DS40M4	44.61	95.73	0.427
pilD	Acinetobacter baumannii D1279779	41.481	96.085	0.399
pilD	Acinetobacter nosocomialis M2	41.111	96.085	0.395