Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JJN14_RS00140 Genome accession   NZ_CP067992
Coordinates   25674..27011 (+) Length   445 a.a.
NCBI ID   WP_236253694.1    Uniprot ID   -
Organism   Streptococcus mitis strain S022-V7-A3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20674..32011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJN14_RS00110 (JJN14_00110) comW 22069..22305 (+) 237 WP_201058582.1 sigma(X)-activator ComW Regulator
  JJN14_RS00115 (JJN14_00115) - 22536..23822 (+) 1287 WP_001832534.1 adenylosuccinate synthase -
  JJN14_RS00120 (JJN14_00120) tadA 24022..24489 (+) 468 WP_201058583.1 tRNA adenosine(34) deaminase TadA -
  JJN14_RS00130 (JJN14_00130) - 24676..25119 (+) 444 WP_084926802.1 dUTP diphosphatase -
  JJN14_RS00135 (JJN14_00135) - 25070..25636 (+) 567 WP_201058584.1 histidine phosphatase family protein -
  JJN14_RS00140 (JJN14_00140) radA 25674..27011 (+) 1338 WP_236253694.1 DNA repair protein RadA Machinery gene
  JJN14_RS00145 (JJN14_00145) - 27082..27777 (+) 696 WP_201058585.1 TIGR00266 family protein -
  JJN14_RS00150 (JJN14_00150) - 27937..28434 (+) 498 WP_201058586.1 beta-class carbonic anhydrase -
  JJN14_RS00155 (JJN14_00155) - 28459..29274 (+) 816 WP_201058587.1 PrsW family intramembrane metalloprotease -
  JJN14_RS00160 (JJN14_00160) - 29420..30388 (+) 969 WP_201058588.1 ribose-phosphate diphosphokinase -
  JJN14_RS00165 (JJN14_00165) - 30506..30943 (+) 438 WP_201058589.1 CoA-binding protein -
  JJN14_RS00170 (JJN14_00170) - 30975..31985 (-) 1011 WP_201058590.1 YeiH family protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 48629.89 Da        Isoelectric Point: 5.5963

>NTDB_id=526120 JJN14_RS00140 WP_236253694.1 25674..27011(+) (radA) [Streptococcus mitis strain S022-V7-A3]
MCQNCEYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPG
SLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVERIQPNFLI
IDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGERHHTFRILR
AVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDF
NRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIRRVNRIEQR
INEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFS

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=526120 JJN14_RS00140 WP_236253694.1 25674..27011(+) (radA) [Streptococcus mitis strain S022-V7-A3]
GTGTGTCAAAATTGTGAATATAATTCGCCTAAGTATCTAGGACGTTGTCCTAACTGTGGGTCTTGGTCTTCTTTTGTAGA
AGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAACCAAGCCCATGAAACTGGCTGAGG
TGACTTCCATCAATGTCAATCGAACTAAGACAGAGATGGAGGAATTTAACCGTGTACTTGGAGGCGGAGTGGTACCAGGA
AGTCTCGTCCTCATCGGTGGGGATCCTGGAATCGGGAAATCAACGCTTCTACTACAAGTCTCAACCCAGTTGTCCCAAGT
GGGAACGGTTCTCTACGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTGCAGAGCGTTTGGGTGATATTGATA
GCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGAGCTGAGGTAGAAAGAATCCAGCCAAACTTCCTCATT
ATTGACTCTATCCAGACCATTATGTCTCCTGAGATTTCAGGGGTACAGGGGTCTGTTTCTCAGGTGCGTGAGGTGACTGC
TGAACTTATGCAATTAGCGAAGACTAATAACATTGCTATCTTTATCGTCGGCCATGTGACCAAGGAAGGAACCTTGGCTG
GTCCACGTATGTTGGAGCATATGGTGGATACAGTGCTTTACTTTGAAGGGGAGCGTCACCATACCTTTCGTATTTTGAGA
GCGGTCAAAAATCGTTTTGGTTCTACCAATGAAATTGGGATTTTTGAGATGCAGTCGGGTGGCTTGGTTGAAGTCCTCAA
TCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGAGCGACTGGCTCATCAATCGTTGTGACCATGGAAGGGACGCGTC
CGATTTTAGCAGAGGTTCAGGCCTTGGTGACGCCAACCATGTTTGGAAATGCCAAGCGCACGACCACAGGTCTGGACTTC
AATCGTGCGAGTCTGATTATGGCTGTTTTGGAAAAGCGTGCAGGGCTTCTCTTGCAAAATCAGGATGCCTATCTCAAATC
TGCTGGTGGTGTCAAATTGGATGAACCTGCCATTGACTTAGCCGTCGCTGTTGCTATTGCTTCGAGCTACAAGGACAAGC
CAACCAACCCTCAGGAATGTTTTGTGGGAGAACTTGGTTTGACGGGAGAAATTCGGCGCGTGAATCGTATCGAGCAGCGT
ATTAATGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCCAAAAATTCCTTGACAGGAATCACTCCACCTAAGGA
AATTCAGGTTATTGGGGTGACAACGATTCAGGAAGTCTTGAAAAAGGTATTCTCCTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(352-417)

AAA domain (AAA_25)

(55-210)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(2-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

98.876

100

0.989
  radA Streptococcus mitis NCTC 12261

98.876

100

0.989
  radA Streptococcus pneumoniae Rx1

98.876

100

0.989
  radA Streptococcus pneumoniae D39

98.876

100

0.989
  radA Streptococcus pneumoniae R6

98.876

100

0.989
  radA Streptococcus pneumoniae TIGR4

98.876

100

0.989
  radA Bacillus subtilis subsp. subtilis str. 168

62.921

100

0.629