Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   K6O12_RS21075 Genome accession   NZ_CP082075
Coordinates   4361759..4363957 (-) Length   732 a.a.
NCBI ID   WP_001468126.1    Uniprot ID   -
Organism   Escherichia coli strain MS20658     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4356759..4368957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6O12_RS21055 (K6O12_20940) hslU 4357563..4358894 (-) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -
  K6O12_RS21060 (K6O12_20945) hslV 4358904..4359434 (-) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  K6O12_RS21065 (K6O12_20950) ftsN 4359527..4360486 (-) 960 WP_000068834.1 cell division protein FtsN -
  K6O12_RS21070 (K6O12_20955) cytR 4360578..4361603 (-) 1026 WP_000644911.1 DNA-binding transcriptional regulator CytR Regulator
  K6O12_RS21075 (K6O12_20960) priA 4361759..4363957 (-) 2199 WP_001468126.1 primosomal protein N' Machinery gene
  K6O12_RS21080 (K6O12_20965) rpmE 4364160..4364372 (+) 213 WP_000710769.1 50S ribosomal protein L31 -
  K6O12_RS21085 (K6O12_20970) yiiX 4364433..4365041 (-) 609 WP_000702302.1 YiiX family permuted papain-like enzyme -
  K6O12_RS21090 (K6O12_20975) metJ 4365101..4365418 (-) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  K6O12_RS21095 (K6O12_20980) metB 4365695..4366855 (+) 1161 WP_000197201.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81758.06 Da        Isoelectric Point: 9.1153

>NTDB_id=525412 K6O12_RS21075 WP_001468126.1 4361759..4363957(-) (priA) [Escherichia coli strain MS20658]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGYRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSSLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPVLLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMHLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=525412 K6O12_RS21075 WP_001468126.1 4361759..4363957(-) (priA) [Escherichia coli strain MS20658]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTATCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGCG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTGTTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCGCCAAAGCAACAACAGGCACTGGCGGCATTGCGGCAAGGCAAAATCTGGCGCGACCAAGTTGCCACGCTTGAATTT
AATGATGCCGCGTTACAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAAGTTTATCTCAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTAGAAGTTCTGCATTCCAGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGTTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTTTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACACCCGCGCTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGTCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCGGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGTCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGTGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTGCCGGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGCTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAACAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCATTACTGGACGTAGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGCTTTACACCCAAGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGTGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACATTGCTGTATAAAGGCT
ATGACGCCTTTGCAGAACAGGCGCTTGCCGAGCGGCGAATGATGCATTTGCCGCCGTGGACCAGTCATGTGATTGTGCGC
GCCGAAGATCATAACAATCAACATGCACCACTGTTCCTGCAACAGTTGCGAAATCTCATCCTCTCCAGCCCACTGGCGGA
TGACAAGCTCTGGGTACTCGGCCCGGTTCCGGCACTGGCCCCTAAGCGTGGTGGCCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

DEAD/DEAH box helicase (DEAD)

(203-362)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.776

100

0.451