Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   K6134_RS20835 Genome accession   NZ_CP082045
Coordinates   4288029..4290227 (-) Length   732 a.a.
NCBI ID   WP_001350873.1    Uniprot ID   B7MI67
Organism   Escherichia coli strain MS21324     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4283029..4295227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6134_RS20815 (K6134_20680) hslU 4283833..4285164 (-) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -
  K6134_RS20820 (K6134_20685) hslV 4285174..4285704 (-) 531 WP_000208235.1 ATP-dependent protease subunit HslV -
  K6134_RS20825 (K6134_20690) ftsN 4285797..4286756 (-) 960 WP_000068834.1 cell division protein FtsN -
  K6134_RS20830 (K6134_20695) cytR 4286848..4287873 (-) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  K6134_RS20835 (K6134_20700) priA 4288029..4290227 (-) 2199 WP_001350873.1 primosomal protein N' Machinery gene
  K6134_RS20840 (K6134_20705) rpmE 4290430..4290642 (+) 213 WP_000710769.1 50S ribosomal protein L31 -
  K6134_RS20845 (K6134_20710) yiiX 4290703..4291311 (-) 609 WP_000702319.1 YiiX family permuted papain-like enzyme -
  K6134_RS20850 (K6134_20715) metJ 4291371..4291688 (-) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  K6134_RS20855 (K6134_20720) metB 4291965..4293125 (+) 1161 WP_000197200.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81767.07 Da        Isoelectric Point: 9.1266

>NTDB_id=524810 K6134_RS20835 WP_001350873.1 4288029..4290227(-) (priA) [Escherichia coli strain MS21324]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQRKIWRDQVAELEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=524810 K6134_RS20835 WP_001350873.1 4288029..4290227(-) (priA) [Escherichia coli strain MS21324]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGAGTCGCGTGCGCGTGCCGTTTGGCAAACAACAGGAGCGTATCGGGGTTGTGGTGTCAGTGAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCTGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCGCTGGCAGCGTTACGGCAACGCAAAATCTGGCGTGATCAGGTCGCGGAACTTGAATTT
AATGATGCCGCGTTGCAGGCACTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGGTTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCGGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGCCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGTGGTTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGCCACCATTGCGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCTTGCGGT
TCCACGCACCTGGTCCCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAACAACTGGCGGAAGTACATCGCGGTGGCGCGA
GGATTTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCATTACTGGACGTGGAC
GGCGCGCTGTTTTCTGCCGATTTCCGCTCGGCGGAGCGTTTTGCCCAGCTTTACACCCAAGTGGCAGGCCGTGCCGGACG
TGCCGGTAAACAGGGCGAAGTAGTTCTGCAAACGCACCATCCTGAACATCCGCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCACTGGCTGAACGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTCATTCTCGCCAGCCCACTGGCAGA
CGAGAAACTGTGGGTTCTCGGTCCGGTTCCGGCGCTGGCCCCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTGCTGGATGTCGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

DEAD/DEAH box helicase (DEAD)

(203-362)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7MI67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

45.183

100

0.455