Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JHX84_RS13565 Genome accession   NZ_CP067043
Coordinates   2622469..2623539 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain 19573-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2617469..2628539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHX84_RS13535 (JHX84_13530) - 2617944..2618189 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  JHX84_RS13540 (JHX84_13535) - 2618426..2618806 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JHX84_RS13550 (JHX84_13545) - 2619012..2619842 (+) 831 WP_003153080.1 STAS domain-containing protein -
  JHX84_RS13555 (JHX84_13550) - 2619880..2621181 (-) 1302 WP_014305416.1 hemolysin family protein -
  JHX84_RS13560 (JHX84_13555) - 2621327..2622277 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  JHX84_RS13565 (JHX84_13560) comGA 2622469..2623539 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  JHX84_RS13570 (JHX84_13565) comGB 2623526..2624563 (+) 1038 WP_003153086.1 competence type IV pilus assembly protein ComGB Machinery gene
  JHX84_RS13575 (JHX84_13570) comGC 2624568..2624876 (+) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  JHX84_RS13580 (JHX84_13575) comGD 2624866..2625303 (+) 438 WP_003153088.1 competence type IV pilus minor pilin ComGD Machinery gene
  JHX84_RS13585 (JHX84_13580) comGE 2625287..2625601 (+) 315 WP_046341385.1 competence type IV pilus minor pilin ComGE -
  JHX84_RS13590 (JHX84_13585) comGF 2625615..2626010 (+) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  JHX84_RS13595 (JHX84_13590) comGG 2626011..2626388 (+) 378 WP_003153092.1 competence type IV pilus minor pilin ComGG Machinery gene
  JHX84_RS13600 (JHX84_13595) - 2626445..2626624 (+) 180 WP_003153093.1 YqzE family protein -
  JHX84_RS13605 (JHX84_13600) - 2626664..2626993 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  JHX84_RS13610 (JHX84_13605) tapA 2627252..2627923 (+) 672 WP_046341384.1 amyloid fiber anchoring/assembly protein TapA -
  JHX84_RS13615 (JHX84_13610) sipW 2627895..2628479 (+) 585 WP_003153100.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=524653 JHX84_RS13565 WP_003153083.1 2622469..2623539(+) (comGA) [Bacillus velezensis strain 19573-3]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=524653 JHX84_RS13565 WP_003153083.1 2622469..2623539(+) (comGA) [Bacillus velezensis strain 19573-3]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374