Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BTG_RS27580 Genome accession   NC_018500
Coordinates   5445880..5446923 (+) Length   347 a.a.
NCBI ID   WP_001013183.1    Uniprot ID   A0A9X6VF82
Organism   Bacillus thuringiensis HD-771     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5440880..5451923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTG_RS27545 (BTG_27885) - 5441246..5441734 (+) 489 WP_000764268.1 hypothetical protein -
  BTG_RS27550 (BTG_27890) - 5441769..5442080 (-) 312 WP_001093238.1 hypothetical protein -
  BTG_RS27555 (BTG_27895) - 5442334..5442549 (+) 216 WP_001008325.1 DUF3912 family protein -
  BTG_RS36595 - 5442637..5443028 (+) 392 Protein_5477 hypothetical protein -
  BTG_RS27565 (BTG_27910) - 5443108..5444502 (-) 1395 WP_001094344.1 L-cystine transporter -
  BTG_RS27570 (BTG_27915) - 5444610..5444852 (-) 243 WP_000375619.1 DUF2626 domain-containing protein -
  BTG_RS27575 (BTG_27920) - 5444979..5445674 (-) 696 WP_000434110.1 helix-turn-helix transcriptional regulator -
  BTG_RS27580 (BTG_27925) comGA 5445880..5446923 (+) 1044 WP_001013183.1 competence type IV pilus ATPase ComGA Machinery gene
  BTG_RS27585 (BTG_27930) comGB 5446910..5447947 (+) 1038 WP_025989085.1 competence type IV pilus assembly protein ComGB -
  BTG_RS27590 (BTG_27935) comGC 5447959..5448258 (+) 300 WP_001178680.1 competence type IV pilus major pilin ComGC -
  BTG_RS27595 (BTG_27940) comGD 5448255..5448710 (+) 456 WP_002082168.1 competence type IV pilus minor pilin ComGD -
  BTG_RS27600 (BTG_27945) - 5448703..5449005 (+) 303 WP_000829465.1 hypothetical protein -
  BTG_RS27605 (BTG_27950) - 5448975..5449157 (+) 183 Protein_5486 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BTG_RS27610 (BTG_27955) - 5449306..5450475 (-) 1170 WP_000190216.1 site-specific integrase -
  BTG_RS27615 (BTG_27960) - 5450678..5451022 (-) 345 WP_000847493.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39196.60 Da        Isoelectric Point: 8.8958

>NTDB_id=52456 BTG_RS27580 WP_001013183.1 5445880..5446923(+) (comGA) [Bacillus thuringiensis HD-771]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LRIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEANGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=52456 BTG_RS27580 WP_001013183.1 5445880..5446923(+) (comGA) [Bacillus thuringiensis HD-771]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGATATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCGAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGTATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTTACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAATGTCCGTTTTGCAGAGGGAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGGTATGAGTTAAAACAAGCGCTTAAAGAAGCGAATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGGTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment