Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JH255_RS05340 Genome accession   NZ_CP066996
Coordinates   1259231..1260433 (-) Length   400 a.a.
NCBI ID   WP_227971345.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 33-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1254231..1265433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH255_RS05310 (JH255_05270) - 1254734..1255225 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH255_RS05315 (JH255_05275) - 1255222..1255512 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  JH255_RS05320 (JH255_05280) - 1255587..1255988 (-) 402 WP_040940795.1 SymE family type I addiction module toxin -
  JH255_RS05330 (JH255_05290) coaE 1257724..1258347 (-) 624 WP_040940796.1 dephospho-CoA kinase -
  JH255_RS05335 (JH255_05295) - 1258361..1259224 (-) 864 WP_129588889.1 A24 family peptidase -
  JH255_RS05340 (JH255_05300) pilC 1259231..1260433 (-) 1203 WP_227971345.1 type II secretion system F family protein Machinery gene
  JH255_RS05345 (JH255_05305) - 1260859..1261275 (+) 417 WP_227971328.1 pilin -
  JH255_RS05350 (JH255_05310) - 1261364..1261774 (+) 411 WP_274316877.1 pilin -
  JH255_RS05355 (JH255_05315) pilB 1261814..1263547 (+) 1734 WP_274497634.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH255_RS05360 (JH255_05320) pilR 1263965..1265359 (-) 1395 WP_274497633.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 43582.64 Da        Isoelectric Point: 10.1730

>NTDB_id=524042 JH255_RS05340 WP_227971345.1 1259231..1260433(-) (pilC) [Xanthomonas campestris pv. campestris strain 33-2]
MSPFVWEGTDKRGVKMKGEQTARNANLLRAELRRQGITPIVVKPKPKPLFGAAGKKVTPKDIAFFSRQMATMMKSGVPIV
GALEIIGSGQKNPRMRNMVGQVRTDIEGGSSLHEAISRHPVQFDELYRNLVKAGEGAGVLETVLDTIASYKENIEALKGK
IKKALFYPAMVMAVALLVSSILLIWVVPQFEDVFKGFGAELPAFTQLIVNASRFMVVWWWALLVVLIGAIVGFIFAYKRS
PAMQHGMDRLILKVPIIGQIMHNSSIARFARTTAVTFKAGVPLVEALSIVAGATGNSVYEAAVLRMRDDVSVGYPVNVAM
KQVNLFPHMVVQMTAIGEEAGALDAMLFKVAEYFEQEVNNAVDALSSLIEPLIMVFIGTIVGGMVIGMYLPIFKLASVVG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=524042 JH255_RS05340 WP_227971345.1 1259231..1260433(-) (pilC) [Xanthomonas campestris pv. campestris strain 33-2]
ATGAGTCCGTTTGTCTGGGAAGGGACCGACAAGCGTGGCGTGAAGATGAAAGGGGAGCAGACTGCTCGAAATGCAAACTT
GCTGCGGGCCGAGTTGCGCCGCCAAGGCATCACCCCGATAGTGGTGAAGCCTAAGCCGAAACCGCTCTTCGGCGCAGCAG
GCAAGAAGGTCACCCCCAAGGACATCGCTTTTTTCAGCAGACAGATGGCGACGATGATGAAGTCTGGCGTGCCCATCGTT
GGAGCGCTGGAAATCATTGGCAGCGGCCAGAAAAATCCGCGTATGCGGAACATGGTCGGTCAAGTTCGCACCGACATCGA
GGGCGGCTCTTCGCTGCACGAGGCTATTAGCAGGCACCCTGTCCAGTTCGATGAGCTTTATCGAAATCTTGTCAAGGCGG
GCGAAGGCGCTGGCGTCTTGGAAACGGTGCTCGATACCATTGCTTCTTACAAAGAAAACATCGAAGCACTGAAAGGCAAG
ATCAAGAAGGCATTGTTTTATCCCGCCATGGTGATGGCAGTCGCCTTACTTGTCAGCTCCATCCTTTTGATTTGGGTAGT
CCCACAGTTTGAGGACGTCTTCAAAGGATTCGGGGCAGAACTTCCTGCCTTCACTCAGCTGATCGTGAATGCATCACGTT
TTATGGTGGTGTGGTGGTGGGCCCTTTTGGTTGTCCTGATTGGAGCAATCGTAGGTTTTATTTTTGCTTACAAACGCTCT
CCGGCAATGCAGCACGGGATGGACAGACTGATTCTTAAAGTGCCGATCATTGGTCAGATCATGCACAACAGCTCCATTGC
CCGCTTTGCTAGAACCACCGCTGTAACATTTAAGGCTGGTGTACCGCTTGTTGAAGCACTCAGCATCGTTGCCGGCGCAA
CAGGCAACTCGGTCTACGAGGCTGCAGTCCTGCGCATGCGCGACGACGTGTCCGTGGGCTACCCGGTCAACGTGGCGATG
AAGCAGGTCAACCTGTTCCCGCACATGGTGGTACAGATGACCGCCATCGGCGAAGAGGCCGGCGCCTTGGATGCAATGCT
GTTCAAAGTTGCCGAGTACTTCGAGCAAGAGGTCAACAATGCGGTTGATGCGCTGAGCAGCCTGATCGAACCCCTCATCA
TGGTGTTCATTGGTACCATCGTCGGCGGCATGGTCATCGGCATGTACCTGCCCATCTTCAAGCTCGCTTCGGTGGTTGGA
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(65-188)

Type II secretion system (T2SS), protein F (T2SSF)

(269-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baylyi ADP1

52.5

100

0.525
  pilC Pseudomonas stutzeri DSM 10701

52.513

99.5

0.523
  pilC Legionella pneumophila strain ERS1305867

51.768

99

0.513
  pilC Acinetobacter baumannii D1279779

50.5

100

0.505
  pilG Neisseria gonorrhoeae MS11

43.216

99.5

0.43
  pilG Neisseria meningitidis 44/76-A

42.714

99.5

0.425
  pilC Vibrio cholerae strain A1552

41

100

0.41
  pilC Vibrio campbellii strain DS40M4

39.295

99.25

0.39