Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdJ   Type   Machinery gene
Locus tag   BN140_RS01325 Genome accession   NC_018227
Coordinates   271088..272011 (+) Length   307 a.a.
NCBI ID   WP_014866163.1    Uniprot ID   -
Organism   Methanoculleus bourgensis MS2     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 266088..277011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN140_RS01305 (BN140_0259) hisI 266521..266886 (-) 366 WP_014866159.1 phosphoribosyl-AMP cyclohydrolase -
  BN140_RS01310 (BN140_0260) - 266893..267696 (-) 804 WP_014866161.1 A24 family peptidase C-terminal domain-containing protein -
  BN140_RS01315 (BN140_0261) - 268040..268678 (+) 639 WP_048104419.1 hypothetical protein -
  BN140_RS01320 (BN140_0262) epdL 268665..271091 (+) 2427 WP_014866162.1 type II/IV secretion system ATPase subunit Machinery gene
  BN140_RS01325 (BN140_0263) epdJ 271088..272011 (+) 924 WP_014866163.1 type II secretion system F family protein Machinery gene
  BN140_RS01330 (BN140_0264) epdK 272014..273051 (+) 1038 WP_014866164.1 type II secretion system F family protein Machinery gene
  BN140_RS01335 (BN140_0265) - 273136..274629 (-) 1494 WP_024265328.1 cobyric acid synthase -
  BN140_RS01340 (BN140_0266) - 274688..276334 (+) 1647 WP_014866165.1 adenosylcobalamin-dependent ribonucleoside-diphosphate reductase -
  BN140_RS01345 (BN140_0267) yciH 276377..276682 (+) 306 WP_014866167.1 stress response translation initiation inhibitor YciH -

Sequence

Protein


Download         Length: 307 a.a.        Molecular weight: 34352.19 Da        Isoelectric Point: 7.9010

>NTDB_id=52290 BN140_RS01325 WP_014866163.1 271088..272011(+) (epdJ) [Methanoculleus bourgensis MS2]
MNSYERFCFNLIGRDLKKKRGDFISLRKDLMGARMNTPFEAYLATAYVSSVVVGLAAAVLIGLFTYLLRVPEMITYRGAV
PEFFYALNDYKLVIGTIIITVLSLLIFGGISYLIFLLYPGIRAGERRRNIDATLPYAINYVTAMSSAGITPDEVFRLLGQ
SKIYGESAVEARYVSRETDFFGKDLLEALRTVSQATPSERMREFLQGAVASISSGSNLTEYFRTKAHQYTLENNQQQKTF
LETLGLIAESYVTAMVAGMLFLIILQSVMTILSGDSNPLFLYIIIYLIVPFGSMMFVILISSMTPEV

Nucleotide


Download         Length: 924 bp        

>NTDB_id=52290 BN140_RS01325 WP_014866163.1 271088..272011(+) (epdJ) [Methanoculleus bourgensis MS2]
ATGAACAGTTATGAGCGGTTCTGCTTCAACCTGATCGGCCGGGACTTAAAGAAGAAGCGGGGGGACTTCATCAGCCTCCG
CAAGGATCTCATGGGGGCCCGGATGAATACGCCGTTTGAGGCTTACCTTGCGACCGCCTACGTCTCCTCGGTTGTCGTGG
GGCTGGCCGCCGCCGTGCTCATCGGGCTCTTTACCTACCTCCTGAGGGTCCCTGAGATGATCACCTACCGGGGGGCGGTC
CCTGAGTTTTTCTACGCGTTGAACGATTACAAACTCGTGATCGGCACCATCATCATCACGGTCCTCTCGCTCCTGATCTT
CGGCGGCATATCCTACCTGATCTTCCTCCTCTATCCCGGCATACGGGCTGGAGAGCGTCGCCGGAACATCGACGCCACCC
TGCCCTACGCCATCAACTACGTCACCGCGATGTCCTCGGCAGGGATCACCCCCGACGAGGTCTTCCGGCTGCTCGGCCAG
AGTAAGATCTACGGCGAGAGCGCCGTTGAGGCGCGTTACGTCTCCCGGGAGACCGACTTCTTCGGGAAGGACCTCCTCGA
AGCCCTGCGGACGGTCTCGCAGGCGACGCCGAGCGAACGGATGCGGGAGTTCCTGCAGGGGGCCGTTGCGAGCATCTCGA
GCGGGAGCAACCTCACAGAGTATTTCCGCACGAAGGCTCACCAGTATACGCTTGAGAACAACCAGCAGCAGAAGACGTTC
CTCGAGACGCTCGGGTTGATCGCGGAGTCCTACGTCACCGCGATGGTTGCGGGTATGCTCTTCCTGATCATCCTGCAGTC
GGTGATGACAATCCTCTCCGGTGACTCGAATCCGCTCTTCCTCTACATCATCATCTACCTGATCGTGCCGTTCGGGAGCA
TGATGTTCGTCATCCTGATCAGTTCCATGACCCCGGAGGTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(138-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdJ Methanoculleus thermophilus strain DSM 2373

91.857

100

0.919

Multiple sequence alignment