Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   T1E_RS01075 Genome accession   NC_018220
Coordinates   248074..249336 (-) Length   420 a.a.
NCBI ID   WP_128573554.1    Uniprot ID   -
Organism   Pseudomonas putida DOT-T1E     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 243074..254336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  T1E_RS01060 (T1E_0208) - 244740..246341 (-) 1602 WP_003249151.1 AAA family ATPase -
  T1E_RS01065 (T1E_0209) aroB 246357..247454 (-) 1098 WP_003249149.1 3-dehydroquinate synthase -
  T1E_RS01070 (T1E_0210) aroK 247551..248069 (-) 519 WP_003249147.1 shikimate kinase AroK -
  T1E_RS01075 (T1E_0211) pilQ 248074..249336 (-) 1263 WP_128573554.1 secretin N-terminal domain-containing protein Machinery gene
  T1E_RS01080 (T1E_0212) - 249260..250261 (-) 1002 WP_004575491.1 pilus assembly protein PilP -
  T1E_RS01085 (T1E_0213) - 250273..250809 (-) 537 WP_014859127.1 PilN domain-containing protein -
  T1E_RS01090 (T1E_0214) pilM 250806..251687 (-) 882 WP_014859128.1 pilus assembly protein PilM -
  T1E_RS01095 (T1E_0215) - 251891..252079 (+) 189 Protein_214 hypothetical protein -
  T1E_RS01100 (T1E_0216) pyk 252146..253600 (-) 1455 WP_003251912.1 pyruvate kinase -
  T1E_RS01105 (T1E_0217) - 253781..254167 (+) 387 WP_014859130.1 hypothetical protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45757.49 Da        Isoelectric Point: 5.0871

>NTDB_id=52230 T1E_RS01075 WP_128573554.1 248074..249336(-) (pilQ) [Pseudomonas putida DOT-T1E]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGGISTGSLSVDERT
NTLVVYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSRIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=52230 T1E_RS01075 WP_128573554.1 248074..249336(-) (pilQ) [Pseudomonas putida DOT-T1E]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGTAGCGTCACTTTGCGCTTGCAGGACGTGCCGTGGGATCAGGCGCTCGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGACGCGCAGCTGCAGCCATTGCGTCGGGAACTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCGGCATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGTTGGTGGCGCAGTTGGACGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGAGCCGGCAGCCGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGTTGCTGGATCTGGAACTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACTCCGGACAACAAGGTG
ATCATGACCGTCCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.617
  comQ Acinetobacter baylyi ADP1

42.667

100

0.457
  pilQ Acinetobacter baumannii D1279779

40.781

100

0.448
  pilQ Acinetobacter baumannii strain A118

40.781

100

0.448
  pilQ Legionella pneumophila strain Lp02

43.269

99.048

0.429
  pilQ Legionella pneumophila strain ERS1305867

43.269

99.048

0.429
  pilQ Vibrio campbellii strain DS40M4

40.323

100

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.446

98.81

0.41
  pilQ Vibrio cholerae strain A1552

41.446

98.81

0.41

Multiple sequence alignment